银杏愈伤组织诱导与生长的优化试验  

Optimization of the Ginkgo Callus Induction and Growth

作　　者：胡燕梅[1] 夏景波[1] 韩晓红[1] 周全[1] 

机构地区：[1]武汉生物工程学院生物工程系,武汉430415

出　　处：《中国农学通报》2010年第24期47-51,共5页Chinese Agricultural Science Bulletin

基　　金：武汉市高校科学研究资助项目"银杏愈伤组织诱导和培养的研究"(2008K079);湖北省教育厅科学技术研究项目"银杏胚性愈伤组织和悬浮细胞培养的研究"(B20094008)

摘　　要：为获得优良的银杏愈伤组织无性系,采用正交试验探讨了4种外植体、基本培养基、萘乙酸(NAA)、6-苄基嘌呤(6-BA)4因素4水平及银杏幼叶汁和胚乳汁对愈伤组织诱导及生长的影响。结果表明:正交试验显示4种因子影响次序是外植体>基本培养基>NAA>6-BA,其中以子叶为外植体,在培养基SH+NAA3.0mg/L+BA1.5mg/L上愈伤组织生长状态最好,诱导率达到100%和生物量达到345.5g/L;银杏胚乳汁促进愈伤组织生长,其生物量可达到545.5g/L,而银杏叶汁却抑制愈伤组织生长。Ginkgo biloba L. callus induction and growth were studied by orthogonal test of 4 kinds of explants, minimal medium, NAA （NAA）, 6-benzyladenine （6-BA） four factors and four levels, and two other factors of ginkgo leaf juice and endosperm juice in order to obtain good callus clones. The experiment results showed that： the orthogonal test showed the effect sequence of four kinds of factors was explants minimal medium NAA 6-BA for callus induction and growth, of which with the cotyledons as explants in the medium SH ＋ NAA 3.0 mg/L＋BA 1.5 mg/L the callus growth state was the best of the induction rate of 100% and biomass 345.5 g/L; ginkgo endosperm juice could improve the growth and the biomass reached 545.5 g/L, but leaf juice inhibitd the growth of callus.

关 键 词：银杏 正交试验 愈伤组织 银杏叶汁 银杏胚乳汁 

分 类 号：Q946.833[生物学—植物学] Q813.12