Rapid Quantification of Chinese Medicine Zuo Jin Pill via RRLC  

作　　者：LIANG Xu ZHANG Xi XU Zhi-xiu LIU Hui HE Ya-jun LUE Yong-hai ZHANG Wei-dong 

机构地区：[1]School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China, [2]School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, P. R. China, [3]Agilent Technologies Shanghai, Shanghai 200131, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第6期905-909,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.30725045);the Special Program for New Drug Innovation of the Ministry of Science and Technology,China(Nos.2009ZX09311-001 and 2008ZX09101-Z-029);China Postdoctoral Science Foundation(No.20080441304);Shanghai Leading Academic Discipline Project,China(No.B906);the Scientific Foundation of Shanghai City,China(Nos.07DZ19728,09DZ1975700 and 09DZ1971500)

摘　　要：A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.

关 键 词：Zuo Jin Pill Rapid resolution liquid chromatography Simultaneous analysis Quality control Hierarchical cluster analysis 

分 类 号：O657.72[理学—分析化学] S853.91[理学—化学]