Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve  

作　　者：ZHANG Di GUO Lei ZHU Na SU Wei-heng GUAN Xin-gang YI Fei HAO Feng XIAO Ying-hong LIU Jia LIU Tian-zhou MA Tong-hui 

机构地区：[1]Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China [2]The Second Hospital of Jilin University, Changchun 130041, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第6期955-957,共3页高等学校化学研究（英文版）

基　　金：Supported by the National Basic Research Program of China(No.2009CB521908);the National Natural Science Foundation of China(Nos.30470405,30570864,30670477 and 30770493)and the National Natural Science Fund for Distinguished Young Scholars of China(No.30325011)

摘　　要：We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system.AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR.Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers.Although primary morpholo-gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference,biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve.The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system.AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR.Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers.Although primary morpholo-gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference,biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve.The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.

关 键 词：AQUAPORIN Sciatic nerve myelin GLYCEROL ATP 

分 类 号：Q421[生物学—神经生物学] TS251.52[生物学—生理学]