毛细管电泳-联吡啶钌电化学发光测定利多卡因  

A Capillary Electrophoresis Method for the Analysis of Lidocaine by Electrochemiluminescence with Tris (2,2′-bipyridyl) ruthenium(Ⅱ)

作　　者：孙汉文[1] 问海芳[1] 苏明[2] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]河北化工医药职业技术学院,河北石家庄050026

出　　处：《河北大学学报（自然科学版）》2010年第6期652-656,共5页Journal of Hebei University(Natural Science Edition)

基　　金：教育部博士点学科基金资助项目(20050075003);河北省自然科学基金资助项目(B2008000583)

摘　　要：基于利多卡因对联吡啶钌在铂电极上的电致发光信号有增敏作用,建立了一种测定利多卡因的毛细管电泳-电化学发光分析方法.讨论了磷酸盐缓冲液pH值、浓度、分离电压、检测电位等实验参数对利多卡因分离检测的影响.在优化的实验条件下,利多卡因在1.5～740μmol/L内呈良好线性,检出限为0.1μmol/L.应用此法测定了尿液中利多卡因的含量,回收率为90%～93.5%.A rapid, simple and sensitive method was developed for the determination of lidocaine by capillary electrophoresis with electrochemiluminescence （ECL） detection, which was based on lidocaine enhanced the ECI. intensity of tris （2,2＇-bipyridyl） ruthenium （ Ⅱ ） in PBS medium. Sample solution was injected to separation capillary of 40 cm length （25 μm i. d. ） by electrokinetic injection for 10 s at 10 kV. Under the optimized conditions, the linear range from 1.5 to 740 μmol/L for lidocaine and detection limit （S/N = 3） of 0.1 μmol/L were achieved. The RSD of ECL intensity and the migration time for five continuous injections of 3.7 μmol/L were 4.0% and 0.7%, respectively. This method was successfully applied to lidocaine assay in urine samples with the recovery of 90%-93.5%.

关 键 词：毛细管电泳 电化学发光 利多卡因 

分 类 号：O657.3[理学—分析化学]