一步法同时提取棉铃虫中肠RNA和蛋白质  

One step isolation of RNA and protein from the midgut of Helicoverpa armigera (Hbner)

作　　者：黄晶晶[1] 韩斌[1] 徐晟[1] 沈文飚[1] 沈晋良 

机构地区：[1]南京农业大学生命科学学院 [2]植物保护学院,江苏南京210095

出　　处：《南京农业大学学报》2010年第6期19-22,共4页Journal of Nanjing Agricultural University

基　　金：农业部高毒农药替代试验示范项目((2005)种植业(植保)函7号)

摘　　要：利用一步法从单个棉铃虫(Helicoverpa armigera(Hübner))的中肠样品中分离出RNA和蛋白质。进一步试验证明提取的RNA纯度和完整性较高,能应用于RT-PCR;所获得的棉铃虫中肠蛋白的SDS-PAGE电泳显示,蛋白条带清晰且能应用于Western blot分析。该方法具有简便和快速的特点,并能够实现核酸和蛋白水平上的相互验证。One step method was used to isolate RNA and protein from the single midgut of Helicoverpa armigera （ Hbner） ,separately. Further experiments proved that the extracted RNA displayed good purity and integrity,and could be used for RT-PCR. Meanwhile,the obtained protein separated by using SDS-PAGE had high definition,and could be applied to the Western blot analysis. Overall,the above method is simple,efficient,and is able to achieve the mutual confirmation between nucleic acid and protein level.

关 键 词：一步法 棉铃虫 RNA 蛋白质 

分 类 号：Q966[生物学—昆虫学]