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作 者:何丽斯[1] 夏冰[2] 孟祥静[2] 王春燕[2] 彭峰[2] 汪仁[2]
机构地区:[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省中国科学院植物研究所,江苏南京210014
出 处:《南京农业大学学报》2010年第6期28-32,共5页Journal of Nanjing Agricultural University
基 金:江苏省科技支撑计划项目(BE2008303)
摘 要:以单、复瓣茉莉2年生扦插苗为材料,研究其在2009年南京地区秋冬自然降温过程中低温胁迫所造成的冷害指数变化及多个抗寒生理生化指标的特性。结果表明:在自然降温条件下,单、复瓣茉莉的冷害指数、相对电导率、硫代巴比妥酸反应物含量、脯氨酸含量均显著上升,可溶性蛋白含量先上升后下降;单、复瓣茉莉的超氧化物歧化酶活性呈现先升高后降低的趋势,于11月17日达到最大值,分别为8.04和12.45U·g-1·min-1,过氧化物酶活性于12月20日达到最大值,复瓣茉莉是单瓣茉莉的3.28倍;两栽培品种的根系活力均呈下降趋势。在本试验多次降温过程中复瓣茉莉的耐寒能力略强于单瓣茉莉,复瓣茉莉抗氧化酶活性表现最为突出。The chilling injury parameter and seven kinds of physiological and biochemical parameters of chilling-tolerance were investigated in the 2-year-old cuttings of two cultivars of Jasminum sambac L. ( single petal jasmine and double petal jasmine) ,under the condition of natural cooling in 2009 year,Nanjing City. The results showed that the chilling injury index,the relative elec-trolyte leakage,the thiobarbituric acid reactive substances content,and proline content increased significantly; the soluble protein contents increased firstly and then decreased,in the two cultivars. The superoxide dismutase activity of single petal jasmine and double petal jasmine first increased and then decreased during this process of natural cooling,and reached the maximum value on November 17,which was 8. 04 and 12. 45 U·g -1 ·min-1,respectively. The peroxidase activity of these two cultivars reached the highest level on December 20 and the one of double petal jasmine was 3. 28 times as much as that of single petal jasmine. The root activity of these two cultivars decreased gradually. The comprehensive analysis of those parameters showed that the double petal jasmine had stronger cold tolerance than single petal jasmine,and the change of oxide resistance enzymes activities were especially obvious in double petal jasmine.
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