江苏省小麦禾谷孢囊线虫群体核糖体DNA-ITS区序列分析  

Sequences analysis of rDNA-ITS region of cereal cyst nematodes on wheat from Jiangsu Province

作　　者：王暄[1] 梁中伟[1] 裴世安[1] 乐秀虎[1] 李红梅[1] 彭德良[2] 

机构地区：[1]南京农业大学农业部作物病虫害监测与防控重点开放实验室,江苏南京210095 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《南京农业大学学报》2010年第6期55-62,共8页Journal of Nanjing Agricultural University

基　　金：公益性行业(农业)科研专项(200903040);国家自然科学基金项目(30900939)

摘　　要：对采集自江苏省徐州、宿迁、连云港和盐城4个地区的15个小麦禾谷孢囊线虫群体进行核糖体DNA-ITS区的RFLP分析。结果表明:测定的所有群体均具有相同的酶切图谱,既具有与国外"B"型群体(印度群体)相同的AluⅠ和RsaⅠ酶切图谱,同时也具有中国群体独特的HinfⅠ和Tru9Ⅰ酶切图谱("C"型)。采用Neighbor-Joining法(MEGA4.0)构建的ITS序列系统进化树显示:所有15个江苏群体均聚在小麦禾谷孢囊线虫组(Heterodera avenae group)下的小麦禾谷孢囊线虫复合种群(H.avenae complex)分支内,且多数江苏群体与草地孢囊线虫(H.pratensis)德国和俄罗斯群体的遗传距离相对较近。通过江苏群体与其他近源群体的ITS序列分析比较,河南郑州群体与澳州孢囊线虫(H.australis)的遗传距离较近,青海群体和河南郑州群体在分子遗传水平上具有明显的地域性,而江苏省和我国其他省份的小麦孢囊线虫群体具有丰富的遗传多样性。ITS region of ribosomal DNA was amplified from 15 populations of cereal cyst nematodes （ CCN） on wheat from 4 districts （ Xuzhou,Suqian,Lianyungang and Yancheng） of Jiangsu Province,China. The ITS-RFLP patterns revealed that all populations showed the same fragments after digestion with 8 restriction enzymes. The Jiangsu populations not only obtained the same patterns as the ＂B＂ type （ Indian populations） by AluⅠand RsaⅠdigestions,but also the ＂C＂ type by HinfⅠand Tru9Ⅰ digestions which were specialized for the Chinese populations. The phylogeny trees were constructed by Neighbor-Joining （ MEGA 4. 0） based on ITS sequences and revealed that all of Jiangsu populations were grouped with the species from Heterodera avenae complex,which was one of the branches of the H. avenae group. Most of the Jiangsu populations were genetically closed to the Russian and German populations of H. pratensis. Comparisons of ITS sequences between Jiangsu populations with other CCN populations from China and abroad revealed the close relationship between Zhengzhou populations and H. australis. The regional identities were clearly showed in the populations from Qinghai Province and Zhengzhou,Henan Province. The rich genetic diversities in CCN populations from Jiangsu and other provinces in China were demonstrated in this study.

关 键 词：禾谷孢囊线虫 核糖体DNA ITS区序列 系统发育 

分 类 号：S432.45[农业科学—植物病理学]