猪卵巢发育的组织学观察及磷酸二酯酶3A的表达研究  

Histological observation and expression patterns of phosphodiesterase 3A in the development of porcine ovaries

作　　者：方天[1] 丁威[1,2] 张伟[1] 周波[1] 石放雄[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]江苏句容农林技术职业学校畜牧兽医系,江苏句容212400

出　　处：《南京农业大学学报》2010年第6期101-104,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30771553);国家863计划项目(2008AA101003)

摘　　要：为研究母猪卵巢在不同发育阶段的组织学变化规律和探讨磷酸二酯酶3A(PDE3A)在卵巢发生发育和成熟过程中的生物学意义,选用二花脸母猪胎90日龄及1日龄、20日龄、120日龄和180日龄的母猪卵巢进行组织学观察,并利用RT-PCR检测PDE3A mRNA的表达情况。结果显示:胎90日龄主要由原始卵泡组成;1日龄、20日龄、120日龄卵巢内特征发育明显;180日龄卵巢内的形态特点与120日龄相似。卵巢PDE3A mRNA表达水平在20日龄时与其他各个阶段相比显著降低。提示:PDE3A在猪从原始卵泡募集到初级卵泡形成过程中可能具有生物学作用。In order to investigate the histological changes of the ovary at different developmental stages and explore biological significances of phosphodiesterase 3A （ PDE3A） during the processes of ovarian occurrence,development and maturation,we examined the ovary from Erhualian pigs of 90 days postcoitum,1,20,120 and 180 days postpartum by histology and RT-PCR of PDE3A in the present experiment. The results showed that ovaries at 90 days postcoitum were mainly composed of primordial follicles,and that the development of 1 day postpartum,20,120 and 180 days postpartum ovaries was obvious and morphological characteristics. The expression level of PDE3A mRNA in ovary at the time of 20 days postpartum decreased significantly compared to the other stages. The significant decrease of PDE3A mRNA might imply some biological functions of PDE3A involved in the follicular recruitment in the pig ovary.

关 键 词：二花脸 卵巢 组织学 磷酸二酯酶3A(PDE3A) 

分 类 号：S852.165[农业科学—基础兽医学]