荧光MGB探针检测妊娠期高血压疾病患者分娩后胎盘sFas表达研究  

Study on the expression of soluble Fas in the placenta of pregnancy induced hypertension（PIH） patients by fluorescent MGB probe real-time PCR

作　　者：沈琪[1] 张志[1] 周晓梅 

机构地区：[1]广东医学院附属福田医院妇产科,广东省深圳518033 [2]广东省深圳市福田区妇幼保健院

出　　处：《中国基层医药》2010年第22期3066-3068,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：基金项目：广东省深圳市福田区科技局科技项目（FTWS002）

摘　　要：目的检测妊娠期高血压疾病（妊高征）患者胎盘中可溶性Fas（sFas）表达。方法应用荧光MGB探针技术分别检测34例重度妊高征患者及30例正常妊娠分娩的胎盘sFas表达水平。结果妊高征患者分娩后胎盘sFas的表达为（3．6403±0．9617）明显高于正常分娩后的胎盘表达（1．3273±0．6063）（P〈0．01）。结论妊高征组胎盘sFas的表达高于正常妊娠，sFas可做为围产期评价细胞增殖和凋亡的重要指标。Objective To investigate the the expression of soluble Fas（sFas） in the placenta of pregnancy induced hypertension（PIH） patients after perinatal. Methods Expression of sFas were detected by Fluorescent MGB Probe Real-Time PCR in 34 severe PIH patients and 30 healthy pregnant women served as normal controls. Results Expression of placenta sFas in 34 patients were significantly higher than those in normal controls. Conclusion PIH patients＇ placenta had higher expressiom of sFas;Detcction of sFas may be helpful to value PIH degrade and sFas would be become a indicative markers of cell proliferation and apoptosis during the perinatal period.

关 键 词：高血压 妊娠性 可溶性Fas(sFas) 原位杂交 荧光 

分 类 号：R714[医药卫生—妇产科学]