RP-HPLC同时测定复方丹参片中多种有效成分的含量  

Determination of Multiple Active Components in Complex Prescription of Salvia miltiorrhiza by RP-HPLC

作　　者：游燕[1] 徐国良[2] 张启云[2] 汤喜兰[2] 郑琴[2] 杨明[2] 

机构地区：[1]南昌大学第四附属医院,南昌330003 [2]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2010年第18期50-53,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家十一五支撑计划(2006BAI09B08-01)

摘　　要：目的:采用高效液相色谱,切换波长-梯度洗脱法同时测定复方丹参制剂中9个有效成分的含量。方法:应用反相C18柱;以乙腈-0.1%磷酸溶液作为梯度洗脱流动相,流速1.0 mL.min-1,检测波长281,203,270 nm;柱温30℃。结果:9个成分的峰面积与浓度的线性关系良好(r>0.999 0);回收率为94.87%～104.31%。结论:此方法可作为复方丹参制剂多成分质量控制的标准。Objective ： To develop an HPLC method for determination of nine active components in complex prescription of salvia mihiorrhiza Bunge by wavelength switching. Method：This HPLC assay was performed on a reversed-phase C18 column with gradient elution of acetonitrile and 0. 1% phosphoric acid solution, the flow rate was 1.0 mL·min^-1 ,detecting wavelength was at 281,203 and 270 nm, and column temperature was 30 ℃. Result： Nine components were linear（ r 〉 0. 999 0） in the range of the test concentration, and the recoveries of the method were between 94.87% -104. 31% , respectively. Conclusion： This method is suitable for the quality control of this commonly used complex prescription.

关 键 词：高效液相色谱法 复方丹参片 有效成分 切换波长 

分 类 号：R284.1[医药卫生—中药学]