茵栀黄注射液对体外四氯化碳致肝细胞损伤的保护作用  

Study on Protective Effect of Yinzhihuang Injection on Hepatic Cell Damage Caused by Carbon Tetrachloride in vitro

作　　者：任永申[1,2] 张萍 鄢丹 段慧娟 李正明 肖小河 

机构地区：[1]解放军三○二医院中西医结合肝病诊疗与研究中心,北京100039 [2]成都中医药大学药学院,成都610075

出　　处：《中国实验方剂学杂志》2010年第18期115-117,121,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30701109)

摘　　要：目的:研究茵栀黄注射液对四氯化碳(CCl4)所致大鼠肝细胞损伤的保护作用。方法:采用CCl4熏蒸法造成体外肝细胞损伤模型,观察不同厂家生产的茵栀黄注射液与复方茵陈注射液及其有效成分黄芩苷及香豆素的保护作用。结果:各厂家生产的茵栀黄注射液均能能显著降低丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)及丙二醛(MDA)的含量,提高谷胱甘肽-过氧化物酶(GSH-Px)的活性,降酶作用以复方茵陈注射液样品的作用最强。结论:茵栀黄注射液及其主要有效成分黄芩苷及香豆素对CCl4所致大鼠肝细胞损伤具有保护作用,各厂家正常样品药效作用具有较好的一致性。Objective：To study the protective effect of Yinzhihuang Injection on hepatic cell damage caused by Carbon tetrachloride （CCl4） in vitro. Method： CCl4 fumigation method was adopted to duplicate hepatic cell damage in vitro, and the protective effect of Yinzhihuang Injection and Fufang Yinchen Injection and their main active ingredients, baicalin and coumarin, from different companies were investigated. Result：The ALT, AST and MDA of damaged cells could be decreased and GSH-Px could be elevated by Yinzhihuang Injections from different factories, and the strongest protective effect was originated by Fufang Yinchen Injection. Conclusion： Both Yinzhihuang Injection and their main active ingredients, baicalin and coumarin, could prevent the hepatic cell damage caused by CCl4 in vitro, and the protective effect of normal samples from different companies had good consistency agreed with each other.

关 键 词：茵栀黄注射液 复方茵陈注射液 四氯化碳 肝细胞损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]