正交优选苗药验方隔梨胃炎消提取工艺  

Optimization of Extraction Techniques for Geli Weiyanxiao by Orthogonal Experiment

作　　者：罗春丽[1] 李金玲[1] 刘红昌[1] 王华磊[1] 赵致[1] 

机构地区：[1]贵州大学农学院中药材研究所贵州省药用植物繁育与种植重点实验室,贵阳550025

出　　处：《中国实验方剂学杂志》2010年第18期129-131,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药材现代产业技术体系建设专项(GZCYTX-02)资助;贵州省中药材资源保护与可持续利用研究创新团队([2010]4006);贵州省科技厅重点实验室计划项目([2010]4015)

摘　　要：目的:优选苗药验方治疗慢性胃炎的提取工艺。方法:选取水和乙醇作为提取溶媒,以溶剂浓度、溶剂用量、提取时间、提取次数及药材粉碎粒度作为考察因素,每个因素设立3个水平;并以提取物的镇痛、抗炎作用,浸出物为指标,利用L9(34)正交设计优选提取工艺。结果:水提最佳提取工艺为粉碎粒度为粗颗粒,加10倍量水,提取1 h,提取3次;醇提最佳提取工艺为粉碎粒度为最粗粉,加10倍量乙醇,提取2 h,乙醇浓度为70%,提取2次。考虑到节约成本以及考察指标浸出物、镇痛率、抗炎率的结果显示水提组均优于醇提组;最终确定提取工艺为水提,药材粉碎粒度为粗颗粒,加10倍量水,提取1 h,提取3次。结论:优选的提取工艺稳定,提取物药效显著。Objective：To optimize the extraction techniques of Geli Weiyanxiao. Method：The selection of optimum proess was investigated by L9 （3^4） orthogonal design with the Geli Weiyanxiao as the observation index. Result：Water extraction process： to smash the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Ethanol extraction process： to smash the best size for 10 mesh number, addingl0-fold 70% ethanol, eatracting for 2 times, 2 hours for each extraction ; In consideration of cost-saving and the results of various indexes, the optimal process condition was selected as follows： the best size for coarse particles, plus 10 times the volume of water, from 1 hour, 3 times. Conclusion：The optimal extraction technique for Geli Weiyanxiao is stable and reliable.

关 键 词：隔梨胃炎消 提取工艺 镇痛作用 抗炎作用 

分 类 号：R283.6[医药卫生—中药学]