花脸香蘑菌丝体培养特性的研究  

Effect of Selected Culture Parameters on the Growth of Lepista sordida Mycelium

作　　者：孟玲[1] 王丽华[1] 黄芳[1] 宋爱荣[1] 

机构地区：[1]山东省应用真菌重点实验室,青岛农业大学药用真菌研究所,山东青岛266109

出　　处：《食用菌学报》2010年第3期41-45,共5页Acta Edulis Fungi

基　　金：山东省农业良种产业化项目(编号:2008LZ-008-01D)的部分研究内容

摘　　要：对花脸香蘑（Lepista sordida）菌丝培养基进行了研究。结果表明：在实验范围内,花脸香蘑菌丝体生长最佳碳源为蔗糖,最佳氮源为黄豆粉;无机盐以加入MgSO40.5 g,KH2PO41g（/L）最好;菌丝体在碳氮比20∶1-100∶1范围内均能生长,在20∶1-30∶1时最佳;菌丝培养不必添加维生素;菌丝体在pH7、8、9时生长均较好。经正交试验结果确定花脸香蘑菌丝体生长最佳培养基为（/L）：蔗糖20 g、豆粉6 g、MgSO40.5g、KH2PO4 1g。The effect of selected physical and nutritional parameters on the growth of Lepista sordida mycelium was studied.Sucrose and soymeal were the most favourable carbon and nitrogen sources,respectively for fungal growth.Mycelial growth was recorded at C∶N ratios between 20∶1 and 100∶1 but ratios between 20∶1 and 30∶1,and pH values between 7 and 9,were optimal.Mycelial growth was maximal in a medium consisting of（per liter） 20 g sucrose,6 g soymeal,1.0 g KH2PO4,and 0.5 g MgSO4 without addition of vitamins.

关 键 词：碳源 氮源 无机盐 维生素 PH 

分 类 号：S646[农业科学—蔬菜学]