牛布氏杆菌间接ELISA检测方法的建立  

Development of indirect ELISA for detection of Brucella abortus

作　　者：宫晓炜[1] 邱昌庆[1] 蔺国珍[1] 郑福英[1] 曹小安[1] 王光华[1] 周继章[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室,甘肃兰州730046

出　　处：《中国兽医科学》2010年第6期598-602,共5页Chinese Veterinary Science

基　　金：家畜疫病病原生物学国家重点实验室基金项目(2009)

摘　　要：采用原核表达并纯化的布氏杆菌周质蛋白BP26作为包被抗原,建立了检测牛布氏杆菌血清抗体的间接ELISA(iELISA)方法。用建立的iELISA方法对138份临床奶牛血清样本进行了检测,并与虎红平板凝集试验(RBPT)和套式PCR(nested-PCR)进行了比较。结果表明,所建立的iELISA与RBPT和nes-ted-PCR的符合率分别为97.82%和98.55%。尽管该方法的敏感性略低于nested-PCR,但用iELISA检测病牛血清的阳性率可达93.00%。该iELISA方法可作为一种有效的牛布氏杆菌病血清学诊断的备用方法。An indirect enzyme-linked immunosorbent assay（iELISA） for detecting antibodies to Brucella abortus was established using the purified recombinant protein BP26 of B.obortus expressed in Escherichia coli as coating antigen.Serum samples（n=138） from dairy cow were simultaneously tested by the iELISA,the rose Bengal plate test（RBPT） and the nested-PCR.The coincidence of iELISA with RBPT and nested-PCR was 97.82% and 98.55%,respectively.Although this iELISA method appeared less sensitive than nested-PCR with positive rate of 93% in dairy cows infected by B.abortus.The iELISA could be used as an alternative method for serological diagnosis of brucellosis.

关 键 词：牛布氏杆菌 BP26重组蛋白 间接酶联免疫吸附试验 

分 类 号：S852.614[农业科学—基础兽医学] R446.61[农业科学—兽医学]