亚慢性镉中毒对肝DNA甲基转移酶基因表达的影响  

Effect of subchronic cadmium toxication on expression of DNA methyltransferase gene in liver of chicken

作　　者：傅业全[1,2] 何宝霞 刘晓瑛 王金涛[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]北京农学院城乡发展学院,北京102206 [3]北京市德宝群兴科技有限公司,北京100193 [4]浙江省余姚市畜牧兽医局疫病防治科,浙江余姚315400

出　　处：《中国兽医科学》2010年第6期626-630,共5页Chinese Veterinary Science

基　　金：黑龙江省教育厅科研基金项目(10551038)

摘　　要：为探讨氯化镉(CdCl2)对鸡肝DNA甲基转移酶表达的影响,选择健康50日龄海兰蛋鸡90只,分为3组,每组30只,以含0、140、210mg/kgCdCl2拌料饲喂,分别于试验开始后第20、40和60d时取肝,采用半定量RT-PCR方法检测三种基因表达情况。结果显示,加镉组DNMT1mRNA和DNMT3amRNA的表达均增加,随着攻毒时间的延长,表达量增加明显。但是3组鸡DNMT3bmRNA的表达未见明显变化。表明,一定剂量的CdCl2可通过改变DNMTsmRNA表达水平,影响表观遗传调控,从而诱导毒性效应或肿瘤发生。Ninety hyline white hens aged 50 days were randomly allocated to 3 groups of 30 hens each.In feed,140 mg/kg CdCl2 was added into the low-dosage cadmium（Cd） group,210 mg/kg CdCl2 was added into the high-dosage cadmium group and the hens in the control group fed on the diet without Cd.Individually on day 20,40 and 60 post-treatment,livers of chickens were collected to detect expression of DNMT1 mRNA,DNMT3a mRNA and DNMT3b mRNA by RT-PCR.The fact that cadmium increased the expressions of DNMT1 mRNA and DNMT3a mRNA along with prolongation of time showed that DNA methylation might be involved in the carcinogenic action of cadmium.

关 键 词：亚慢性镉中毒 鸡 肝 DNA甲基转移酶 

分 类 号：S852.659.6[农业科学—基础兽医学] Q503[农业科学—兽医学]