应用基因芯片技术检测参与大肠腺瘤癌变的表皮生长因子受体信号通路的基因  

Study of EGFR Pathway Involved in Colorectal Adenoma-carcinoma Transition by Using Microarray

作　　者：唐慧[1,2] 邹云莲[1,2] 寿涛[1,2] 郭强[1,3] 严新民[1,2] 

机构地区：[1]云南省第一人民医院 [2]昆明医学院附属昆华医院临床基础医学研究所,云南昆明650032 [3]昆明医学院消化内科,云南昆明650032

出　　处：《昆明医学院学报》2010年第11期25-29,52,共6页Journal of Kunming Medical College

基　　金：云南省科技厅社会发展基金资助项目(2008CD203);云南省社会发展科技计划-社会事业发展专项基金资助项目(2009CA009)

摘　　要：目的全面了解参与大肠腺瘤癌变的表皮生长因子受体信号通路(epidermal growth factor receptorpathway,EGFR pathway)基因.方法分别提取正常大肠粘膜、大肠腺瘤、DukesA、DukesB和DukesC-D大肠腺癌组织的总RNA,生物素标记cRNA探针,分别与5张Affymetrix U133 PLUS 2.0基因芯片杂交,GeneScanner 3000扫描,GCOS1.2软件处理杂交信号,将大肠腺瘤与大肠腺癌(包括DukesA,DukesB和DukesC-D大肠腺癌)与正常大肠粘膜组相比较筛选表达差异基因.应用PathwayV1.0软件对以上筛选出的表达差异基因进行通路分析.结果 EGFR信号通路参与正常大肠粘膜→腺瘤→腺癌这一过程(P<0.05),EGFR信号通路中共有包括MAPK,Shc1,SOS1、Grb和c-FOS基因等29个基因在大肠腺瘤癌变过程中有显著的表达差异(P<0.05),这些基因在大肠腺瘤癌变过程中发挥着重要作用.结论 EGFR信号通路中共有29个基因参与大肠腺瘤癌变,对选择EGFR及其下游信号转导因子作为抗肿瘤治疗的重要靶点有一定指导意义.Objective To comprehensively understand the epidermal growth factor receptor pathway（EGFR pathway）genes involved in the colorectal adenoma-carcinoma transition.Methods Total RNA was extracted form tissues of normal human colorectal mucosa,colorectal adenoma,DukesA,B and C-D colorectal cancer respectively,biotin-labled cRNA target probes were hybridized with 5 Affymetrix Human U133 PLUS 2.0 Gene Chips.Signal images were scanned by gene scanner 3 000 and analyzed with GCOS1.2 software.Then differentially expressed genes were screened out by compared colorectal adenoma and colorectal adenocarcinoma（including DukesA,DukesB and DukesC-D colorectal adenocarcinoma）with normal colorectal mucosa.Then pathway analysis of differentially expressed genes sets was performed by using Pathway V1.0 software.Results EGFR pathway participated in the process of colorectal adenoma-carcinoma transition（P0.05）.There were 29 differentially expressed genes of EGFR pathway,including MAPK,Shc1 SOS1,Grb and c-FOS genes,involved in the process of colorectal adenoma-carcinoma transition（P0.05）,these genes played important roles in the carcinogenesis and progression of colorectal carcinoma.Conclusion There are 29 genes of EGFR pathway participate in the colorectal adenoma-carcinoma transition,the results have some guiding significance for selecting EGFR or it＇s downstream signal transduction factors as anti-tumor therapeutic targets.

关 键 词：大肠腺瘤 大肠腺癌 基因芯片 表皮生长因子受体信号通路 

分 类 号：R755.34[医药卫生—皮肤病学与性病学]