鸦胆子素D抗黄瓜绿斑驳花叶病毒的活性  

Activity of Brucein D against CGMMV

作　　者：陈启建[1] 刘莉莉[1] 欧阳明安[1] 林奇英[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2010年第6期566-570,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金(30770089);国家公益性行业(农业)科研专项(200903034);福建省自然科学基金(2009J01069)资助项目

摘　　要：采用半叶枯斑法、叶圆盘法测定了鸦胆子素D对黄瓜绿斑驳花叶病毒(CGMMV)的抑制效果,结果表明,20μg.mL-1的鸦胆子素D对CGMMV的侵染和增殖均有较强的抑制活性,其对CGMMV侵染和复制抑制率分别为92.6%和88.3%;实时荧光定量PCR测定结果表明,20μg.mL-1该化合物对CGMMV复制酶基因、运动蛋白基因和外壳蛋白基因表达的抑制率分别为88.8%、92.1%和90.5%.采用Western blot方法测定的结果表明,鸦胆子素D可显著抑制黄瓜叶片中CGMMV CP和MP蛋白的表达.The activity of Brucein D against Cucumber green mottle mosaic virus（CGMMV） was investigated by local lesion and leaf discs assay,the results indicated that the compound showed a significant inhibitory activity against CGMMV in vitro,with 92.6% inhibitory ratio against the infection of CGMMV and 88.3% against the replication of CGMMV at the concentration of 20 μg·mL-1.While its inhibitory effect on expression levels of CGMMV Rep,MP and CP gene in cucumber leaf discs was examined by real-time quantitative PCR,which showed that the compound obviously suppressed the expression of genes,with 88.8%,92.1% and 90.5% inhibitory ratio at the concentration of 20 μg·mL-1,respectively.In addition,Western blot test results revealed that the compound obviously suppressed the expression of CGMMV CP and MP in cucumber leaves.

关 键 词：鸦胆子素D 黄瓜绿斑驳花叶病毒 抗病毒活性 

分 类 号：S432.41[农业科学—植物病理学]