不同声学微泡对超声介导体外基因转染的作用  

Different Acoustic Microbubbles Mediated by Ultrasound Exposure Enhanced Gene Transfection In Vitro

作　　者：陈智毅[1] 梁琨[2] 谢明星[3] 

机构地区：[1]广州医学院第三附属医院医学超声科,广州市510150 [2]广州医学院第三附属医院医学妇产科,广州市510150 [3]华中科技大学同济医学院附属协和医院超声影像科、湖北省分子影像重点实验室

出　　处：《中国超声医学杂志》2010年第12期1060-1063,共4页Chinese Journal of Ultrasound in Medicine

基　　金：广东省医学科研基金项目(No.A2010270)

摘　　要：目的探讨超声介导基因转染时,不同声学微泡[脂质体微泡(LM)和SonoVue微泡]对体外基因转染的作用及其安全性。方法将红色荧光蛋白基因(DsRed)和LM加入培养的Hela细胞,后行超声辐照(US),对其他4种不同类型的细胞系(HepG_2、Ishikawa、MCF-7和B16-F10)行超声处理,并与PEI介导的基因转染进行比较分析,运用荧光显微镜、流式细胞术评估基因转染率和细胞损伤。结果培养的细胞经LM和超声辐照联合处理后(P+UTMI)),HepG_2、MCF-7的基因转染率略优于Sono Vue微泡转染时(P<0.01),且未发现显著的细胞损伤;不同细胞类型对超声的反应性不同。与非辐照组相比,转染率和死亡率均有不同程度的增加。结论声学微泡对超声介导的体外基月转染有显著的增效作用,为基因治疗提供一种新颖、高效、安全的非病毒基因转染方法。Objective To evaluate the transfection efficiency and safety of different acoustic microbubbles [SonoVue microbubble and liposome microbubble （LM）] in vitro in order to obtain higher transfection efficiency with minimal effect on cell viability and structural integrity of plasmid DNA. Methods Gene transfection （DsRed）and cell viability were evaluated using microscope and flow eytometry（FCM）. The enhancement of SonoVue mierobubble under ultrasound-targeted microbubble destruction （UTMD）condition was analyzed. Enhanced effects of liposome microbub ble （LM）under UTMD conditions were studied. Treatment parameters optimized in HeLa cells were applied in 4 other cell lines （HepG2 ,Ishikawa,MCF-7 and B16 F10）and compared with the transfection of PEI. Results LM and ultrasound exposure increased transfection efficiency in cultured HepG2, MCF7 cells significantly as compared with SonoVue microbubble （P〈0.01）. These experiments also revealed that responses to ultrasound treatment were different for all tested cell lines and dead and transfected cells in the treated groups were different from the non-irradiated groups significantly. Conclusions Acoustic microbubbles by UTMD could enhance effectively without apparently adverse effect. UTMD can be an effective,noninvasive gene transfection method and provide a novel, safe non viral alternative to gene therapy.

关 键 词：超声 微泡 基因传输 声孔作用 

分 类 号：R445.1[医药卫生—影像医学与核医学]