浙江省汉坦病毒基因分子进化分析  

Molecular Evolution Analysis of Hantaviruses in Zhejiang Province

作　　者：姚苹苹[1] 朱函坪[1] 邓小昭[2] 徐芳[1] 谢荣辉[1] 姚晨辉[3] 翁景清[1] 张云[2] 杨占秋[4] 朱智勇[1] 

机构地区：[1]浙江省疾病预防控制中心出血热疫苗研究浙江省重点实验室,杭州310051 [2]南京军区联勤部军事医学研究所,南京210002 [3]大连医科大学,大连116044 [4]武汉大学,武汉430072

出　　处：《病毒学报》2010年第6期465-470,共6页Chinese Journal of Virology

基　　金：国家"863"科技攻关项目(2007AA02Z465);国家自然科学基金(30771861)

摘　　要：为了分析浙江省汉坦病毒分子流行病学的特点,本文应用分子生物学软件对1981～2007年从浙江省不同地区不同宿主分离的12株汉坦病毒的S、M基因序列进行分子进化分析,结果显示:浙江省属汉滩(HTN)型和汉城(SEO)型的混合型疫区,病毒的基因差异和亲缘远近关系主要表现在地区性,而与病毒分离的年代与宿主关系并不大,表现出明显的地理聚集现象,该现象在HTNV中的表现尤为明显,同一地区分离的病毒表现为较高的基因同源性,系统进化树上分布于同一或临近分支。另外发现分离自浙江省建德地区的Gou3和ZJ5株在SEOV进化枝中构成一独立分支,而且与国内外SEOV其他毒株的基因差异均较大,在浙江省建德地区存在着SOEV的特殊亚型病毒。In order to analyze the molecular epidemiology of Hantavirus（HV） in Zhejiang Province,the complete M and S genome sequences of 12 HV strains from different hosts and locations in Zhejiang Province of China during the period of 1981-2007 were ana1yzed on genetic evolution by DNAstar and MEGA 4.0 software in this research.Phylogenetic analyses revealed that HTN and SEO strains were co-circulating in Zhejiang Province,and the difference in sequence similarity and the phylogeny was closely related to the isolated regions,but had no distinct relationship with the isolate year and the host,indicating a relationship between epidemiology of HFRS and the distribution region,especially in HTNV.The isolates in the same region could be assigned in same or near phylogenetic clade sharing high sequence similarity.Interestingly,the Gou3 strain and ZJ5 strain isolated from Jiande region in Zhejiang Province formed a distinct phylogenetic lineage in SEOV clade,and different from the other SEOV variants outside China.We believed that the special SEOV variants were distributed in Jiande region.

关 键 词：肾综合征出血热 汉坦病毒 序列 分子进化分析 

分 类 号：R373.9[医药卫生—病原生物学]