海洛因诱导ATF-3表达并促进神经元凋亡  

Heroin Induces the Up-Regulation of ATF-3 and Promotes Neuronal Apoptosis

作　　者：李泽鸿[1] 郭勇[1] 卢佩彦[1] 竞花兰[1] 林文盈 刘小山[1] 

机构地区：[1]中山大学法医学系,广州510080 [2]广东阳江市公安局刑科所,阳江529500

出　　处：《热带医学杂志》2010年第11期1265-1266,1292,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30860318)

摘　　要：目的观察海洛因成瘾对大鼠小脑颗粒神经元(cerebellar granule neuron)激活转录因子-3(activating transcription factor-3,ATF-3)蛋白表达的影响,研究海洛因中毒导致的神经元损伤机制。方法体外培养原代小脑颗粒神经元并建立海洛因促神经元凋亡模型,用聚合酶链反应和Western免疫印迹技术,检测小脑颗粒神经元凋亡时ATF-3的表达变化。结果 ATF-3在海洛因诱导的神经元凋亡模型中表达明显上调。结论 ATF-3是介导海洛因诱导神经元凋亡的关键因子。Objective To investigate the relationship between the up-regulation of activating transcription factor-3（ATF-3） and the apoptosis of cerebellar granule neuron（CGN） which was induced by heroin.Methods Cultured CGN in vitro to established the heroin induced apoptosis model and evaluate the expression of ATF-3.Results The expression of ATF-3 was increased during heroin induced apoptosis.Conclusion ATF-3 was a key molecular in heroin induced neuronal apoptosis.

关 键 词：海洛因 小脑颗粒神经元 ATF-3 凋亡 

分 类 号：R996[医药卫生—毒理学]