细粒棘球蚴重组铁蛋白（中国株）疫苗诱导抗攻击感染保护力的观察  

Observation on protection by immunization with recombinant Ferritin vaccine of Echinococcus granulosus （Chinese strain）

作　　者：卜阳[1] 李昭宇[1] 罗永云[1] 于晶晶[2] 于辛酉[2] 师志云[2] 马锐[2] 赵巍[2] 

机构地区：[1]宁夏医学院附属医院肝胆外科,750004 [2]宁夏医学院医学遗传学与细胞生物学教研室

出　　处：《中华肝胆外科杂志》2010年第11期861-862,共2页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金项目（30260105）;宁夏自然科学基金项目（NZ0540）

摘　　要：目的 探讨细粒棘球蚴重组铁蛋白疫苗对原头蚴攻击感染的保护性效果.方法 ICR小鼠随机分为3组（每组12只）：A组（实验组）、B组（佐剂＋PBS组）、C组（空白对照组）,A组、B组共免疫3次,每次间隔2周,C组不做任何处理;免疫接种后8周3组均以1100只原头蚴攻击感染,每次免疫前及攻击感染前断尾取血,留取血清;攻击感染后7个月,剖杀小鼠,查检腹腔内包囊数进行统计学分析.结果 实验组与佐剂对照组、空白对照组比较,其结果均具有统计学意义（P＜0.05）,经计算保护力分别为：73%,85%;佐剂组与空白对照组比较,其结果亦具有统计学意义（P＜0.05）,经计算保护力为：42%.结论 细粒棘球蚴重组铁蛋白可诱导小鼠产生部分免疫保护力,可作为＂鸡尾酒＂疫苗的组成部分进一步研究.Objective To investigate protection by immunization with recombinant Ferritin vaccine of Echinococcus granulosus against protoscolices.Methods ICR mice were randomized into 3groups of 12 mice in each.The mice in group A and B were immunized three times with an interval of two weeks and those in group C did nothing.The animals in all the 3 groups were challenged with 1100 protoscolices intraperitoneally on the 8th week.Serum samples were collected before each inoculation and challenge injection.Seven months later, all the mice were killed and examinated for hydatid cysts.Result The number of cysts was significantly lower in the group A than in group B and C （P〈0.05）.The levels of protection afforded were found to be 73% and 85%, respectively.Meanwhile,the number of cysts was markedly lower in group B than in group C（P〈0.05）.The rate of protection afforded was 42%.Conclusion Recombinant Ferritin vaccine of Echinococcus granulosus shows partial immune protection.Therefore, it might be a suitable candidate for cocktail vaccine study in the future.

关 键 词：免疫疗法 细粒棘球蚴 重组铁蛋白疫苗 保护力 

分 类 号：R686[医药卫生—骨科学]