尿道致病性大肠埃希菌感染对人膀胱上皮细胞基因表达谱的影响  

Gene expression profiles in human bladder epithelial cells stimulated with uropathogenic Escherichia coli

作　　者：葛新[2] 陈锦英[1] 姚萍[1] 谷超[1] 赵凤玲[1] 

机构地区：[1]天津医科大学基础医学院微生物学教研室,300070 [2]武警医学院微生物学教研室,天津300162

出　　处：《中华微生物学和免疫学杂志》2010年第11期998-1003,共6页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金（30470096）;教育部高等学校博士学科点专项科研基金（20070062010）

摘　　要：目的 研究尿道致病性大肠埃希菌（UPEC）菌株132与人膀胱上皮EJ细胞的相互作用,分析该菌株感染对EJ细胞基因表达谱的改变.方法 UPEC132感染EJ细胞,用倒置显微镜观察细菌与细胞的黏附,计算黏附率,并通过激光共聚焦显微镜观察UPEC132对细胞的侵袭.感染UPEC132的EJ细胞与未经细菌感染的细胞提取总RNA,用人类全基因组寡核苷酸微阵列芯片分析差异表达基因,并采用RT-PCR对基因芯片数据进行验证.结果 UPEC132能够黏附于EJ细胞表面,黏附率为（73.20±5.26）% 激光共聚焦显微镜观察发现部分细菌位于细胞内部,证实该菌对EJ细胞具有侵袭性.UPEC132感染后的EJ细胞与未经感染的细胞相比,共有28个基因上调,1个基因下调,主要涉及细胞增殖、炎症反应、细胞凋亡等相关基因.结论 UPEC与尿路上皮细胞的相互作用激活宿主细胞内部多种应答反应与信号转导途径,本研究为深入探索UPEC致病机制奠定基础.Objective To investigate the interaction between uropathogenic Escherichia coli （UPEC） and host uroepithelial cells, define the role uroepithelial cells play in initiating and modulating the host response to infection with UPEC strain. Methods The human bladder transitional epithelial EJ cells were evaluated for their capacities to allow the adherence and invasion by UPEC132, a clinical strain isolated from Tianjin, China, and a cDNA microarray for 22 000 human genes was used to identify the gene expression differences between EJ cells infected with UPEC132 and uninfected EJ cells. Results Microscope observation showed that UPEC132 could adhere to EJ cells with the adherence rate of （73.20 ± 5.26）%. And visualization by confocal microscope revealed that this microorganism could be seen within the cells. EJ cells infected with UPEC132 changed mRNA expression of a total of 29 genes, including 28 genes up-regulated and 1 gene down-regulated. Of these, regulators of growth and proliferation, cytokines, and modulators of apoptotic responses were the most prominent. Conclusion The gene expression profiling of EJ cells is affected by the infection of UPEC strain. The differentially expressed genes may contribute to further investigate the interaction of UPEC and uroepithelial cells.

关 键 词：尿道致病性大肠埃希菌 基因表达 膀胱上皮细胞 基因芯片 

分 类 号：R695[医药卫生—泌尿科学]