合肥地区部分儿童乙型肝炎病毒基因型分析  

Children's genotype analysis of hepatitis B virus and its significance in clinical diagnosis in some areas of Hefei

作　　者：程邦宁[1,2] 沈继龙[3] 

机构地区：[1]安徽医科大学儿科临床学院 [2]安徽省立儿童医院检验科,合肥230051 [3]安徽医科大学病原生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2010年第6期774-776,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的调查合肥地区部分儿童乙型肝炎病毒(HBV)基因型分布情况。方法采用荧光定量PCR法测定HBV-DNA,PCR结合Taqman荧光探针技术法检测HBV基因型。结果 53例患者中B型27例(50.9%),C型24例(45.3%),BC混合型2例(3.8%)。C型HBV-DNA水平和HBeAg阳性率分别为(7.38±0.74)log值和79.17%,明显高于B型的(5.48±1.63)log值和23.10%(P<0.05);C型ALT、TBIL的水平均高于B型(P<0.05)。结论合肥地区部分儿童HBV基因型以B、C型为主,B型占优势,C型次之,C型病情重于B型。Objective To investigate the distribution of children＇s hepatitis B virus genotypes in some areas of Hefei and its clinical significance.Methods HBV-DNA were detected to use real-time fluorescence quantitative PCR,HBV genotypes were detected by PCR and Taqman enzyme fluorescent probe technique.Results Of the 53 patients,27（50.9% ） were genotype B,24（45.3% ） were genotype C,2（3.8% ） were mix-infected by BC.The serum level of HBV-DNA and HBeAg positive rate in patients of genotype C was 7.38 ± 0.74 and 79.17% ,which was higher than 5.48 ± 1.63 and 23.10% in those of genotype B（P 0.05） ,and the levels of ALT,TBIL in genotype C samples were also higher than those of genotype B（P 0.05） .Conclusion Genotype B is a dominated children＇s genotype and genotype C is the second in Hefei areas.The damage to liver induced by genotype C is more severe than that of genotype B.

关 键 词：肝炎病毒 乙型/遗传学 基因型 

分 类 号：R373.21[医药卫生—病原生物学] R394.3[医药卫生—基础医学]