STAT3、WWOX与c-myc在非小细胞肺癌组织中的表达及相关性分析  

The expressions of STAT3,WWOX and c-myc in human non small cell lung cancer tissue and correlativity analysis

作　　者：胡福英[1,2] 王乐朋[2] 侯莉莉[2] 殷刚[1] 王鸿程[2] 

机构地区：[1]天门市第一人民医院,湖北天门431700 [2]泸州医学院附属医院呼吸内科,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2010年第12期1203-1205,1209,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省科技厅重点基金资助项目(2006j13-192)

摘　　要：目的:研究信号转导与转录激活因子3(STAT3)、包含氧化还原酶的WW结构域(WWOX)及原癌基因c-myc在非小细胞肺癌(NSCLC)及肺正常组织中的表达以及三者之间的关系。方法:应用免疫组化染色S-P法,检测STAT3、WWOX及c-myc在40例NSCLC组织和20例正常肺组织中的表达情况,并分析其与各个临床病理指标之间的关系。结果:(1)STAT3和c-myc在NSCLC组织中的阳性表达率分别为92.5%和90%,均明显高于肺正常组织(20%和15%,P<0.01);WWOX在NSCLC中的阳性表达率为30%,明显低于肺正常组织(75%,P<0.01)。(2)STAT3、c-myc的表达水平与NSCLC的组织学类型有关,在腺癌组织中的表达明显高于鳞癌组织(P<0.01);与年龄、性别、肿瘤组织的分化程度、肿瘤大小、TNM分期及淋巴结转移无关;WWOX基因的表达与是否有淋巴结转移相关(P<0.01),在有淋巴结转移的肺癌组织中其表达更低或缺失。(3)STAT3与WWOX在NSCLC组织中的表达呈显著负相关(r=-0.443,P<0.01);STAT3与c-myc蛋白,WWOX与c-myc在NSCLC组织中的表达均不相关(分别为r=-0.084,r=0.115)。结论:在NSCLC组织中存在STAT3和c-myc的过度表达,WWOX在NSCLC组织中呈低表达或表达缺失,提示三者可能参与了NSCLC的发生与发展;在NSCLC的发生过程中,STAT3蛋白与WWOX在NSCLC中的表达呈负相关;而STAT3与c-myc、WWOX与c-myc的表达均无相关性,它们二者之间可能是通过两条不同的途径影响肺癌的发生与发展。AIM： To investigate the expression of STAT3,WWOX and c-myc in non small cell lung cancer（NSCLC） and their clinical significance,and study their relation ship.METHODS： Forty resected tissue samples from patients with NSCLC and twenty normal lung tissues were subjected to immunohistochemical staining（SP） for STAT3,WWOX and c-myc.RESULTS：（1）The positive expression rate of STAT3 and c-myc in NSCLC were 92.5% and 90% respectively,obviously higher than normal lung tissues（20% and 15%,P0.01）;The positive expression rate of WWOX was 30%,apparently lower than normal lung tissues（75%,P0.01）.（2） The expression levels of STAT3 and c-myc were related to pathologic types in NSCLC,which in adenocarcinomas were higher than squmous cell carcinomas（P0.01）,which were regardless with tumor size,histodifferentiation level,clinical stage and lymphatic metastasis;The expression of WWOX was ralated to whether lymph node metastasis in NSCLC（P0.01）.（3） There was a negativie correlation between the expressions of STAT3 and WWOX（r=-0.443,P0.01）;But no relationship was found between STAT3 and c-myc overexpression（r=-0.084） and between WWOX and c-myc overexpression（r=0.115）.CONCLUSION： STAT3 and c-myc were overexpressed in human NSCLC.It indicated these proteins may make an important role in occurrence and development of lung cancer.The expressions of WWOX in human NSCLC was lower,which non expression was may related to the invision and metastasis of tumor.In the tumorigenesis of NSCLC,there is no relationship between expression of STAT3 and c-myc,They played independent roles in oncogenesis of NSCLC.There was a negativie correlation between the expressions of STAT3 and WWOX,but no relationship was found between WWOX and c-myc.

关 键 词：非小细胞肺癌 STAT3 WWOX C-MYC 相关性分析 

分 类 号：R392.3[医药卫生—免疫学]