Extraction and Characterization of PHB from Acidiphilium cryptum DX1-1  

作　　者：徐爱玲 夏金兰 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2010年第6期938-943,共6页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation for Distinguished Group (No. 50621063);the National Natural Science Foundation of China (No.50674101);the National Innovation Experiment Program for University Students (No. 81053321)

摘　　要：A new bacterial strain,was designated as strain Acidiphilium cryptum DX1-1,accumulates intracellular poly-β-hydroxybutyrate particles,four methods which have advantages and disadvantages for each were employed to extract PHB.Chloroform-sodium hypochlorite method is the best in extracting PHB form Acidiphilium cryptum DX1-1.The extraction rate reaches 73%,the purification rate is 92% and molecular weight is 326 kg/mol.Then the PHB extracted by this method was analyzed by ultraviolet-visible absorption spectroscopy,fourier transform infrared（FT-IR） and nuclear magnetic resonance（NMR）.The results show that PHB from strain DX1-1 has the same biochemical structure and character with PHB standard.Mass spectrometer（MS） analysis reveals that the long chain of PHB is destroyed when treated by chloroform-sodium hypochlorite.The differential scanning calorimetry（DSC） of PHB shows PHB from Acidiphilium cryptum DX1-1 has low degree of crystallinity which makes the PHB has a wider range of applications.A new bacterial strain,was designated as strain Acidiphilium cryptum DX1-1,accumulates intracellular poly-β-hydroxybutyrate particles,four methods which have advantages and disadvantages for each were employed to extract PHB.Chloroform-sodium hypochlorite method is the best in extracting PHB form Acidiphilium cryptum DX1-1.The extraction rate reaches 73%,the purification rate is 92% and molecular weight is 326 kg/mol.Then the PHB extracted by this method was analyzed by ultraviolet-visible absorption spectroscopy,fourier transform infrared（FT-IR） and nuclear magnetic resonance（NMR）.The results show that PHB from strain DX1-1 has the same biochemical structure and character with PHB standard.Mass spectrometer（MS） analysis reveals that the long chain of PHB is destroyed when treated by chloroform-sodium hypochlorite.The differential scanning calorimetry（DSC） of PHB shows PHB from Acidiphilium cryptum DX1-1 has low degree of crystallinity which makes the PHB has a wider range of applications.

关 键 词：Acidiphilium cryptum PHB EXTRACTION CHARACTERIZATION 

分 类 号：O611.3[理学—无机化学]