猪链球菌2型强毒株05ZYH33转录调控因子Rgg基因敲除突变体与野生株的基因表达差异分析  

Differential Expression Analysis Between Gene Knockout Mutant of Transcriptional Regulator Rgg and Wild Strain of Streptococcus suis 05ZYH33

作　　者：王中胜[1] 曾小涛[1,2] 袁媛[1] 尉研[1] 郑玉玲[1] 姜永强[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《生物技术通讯》2010年第6期812-817,共6页Letters in Biotechnology

摘　　要：目的:为了解猪链球菌2型强毒株05Z33转录调控因子Rgg的调控作用,用基因芯片方法分析野生株与rgg基因敲除突变体之间的差异表达基因。方法:用猪链球菌2型全基因组序列点样制备芯片,将芯片运用于rgg敲除株与野生株的基因表达差异研究,采用定量real-time PCR(qRT-PCR)验证表达谱结果。结果:在突变体中共发现45个基因表达量变化在2倍以上,其中19个基因表达上调,26个基因表达下调。这些基因在细菌毒力、免疫抗原、DNA合成和修复、基础代谢和ABC转运系统等方面起着重要作用。结论:转录调控因子Rgg是一个全局调控因子,但rgg敲除后并不影响猪链球菌的毒力。Objective： To understand the role of transcriptional regulator Rgg of virulent Streptococcus suis type 2 05Z33,gene microarray method was used to analyze differentially expressed genes between the wild strain and the rgg gene knock-out mutant.Methods： The whole genomic sequences of S.suis type 2 were used to print onto glass slides;the microarray was then applied to differential expression analysis between rgg knock-out strain and wild strain;the results were validated with quantitative real time-PCR（qRT-PCR）.Results： The expressions of 45 genes of the mutant were found to change above two times,of which 19 genes were upregulated,26 genes were down-regulated.These genes play an important role in bacterial virulence,immune antigen,DNA synthesis and repair,basic metabolism and other ABC transporter systems.Conclusion： The transcription regulator Rgg is a global regulatory factor,while the virulence of S.suis is not affected after the rgg knock-out.

关 键 词：猪链球菌2型 基因芯片 转录调控因子 

分 类 号：Q78[生物学—分子生物学]