氧化型低密度脂蛋白对可诱导共刺激分子在人外周血T细胞表达的影响  

Effects of oxidized low-density lipoprotein on expression of inducible costimulator in human peripheral blood T cells

作　　者：徐琳[1] 朱肖星[2] 张亚莉[3] 彭智欣[1] 李炳玲[1] 李志梁[4] 邱健[1] 

机构地区：[1]广州军区广州总医院心血管内科,广东广州510010 [2]第四军医大学干休所,陕西西安710032 [3]贵阳医学院医学检验系,贵州贵阳550004 [4]南方医科大学珠江医院心血管内科,广东广州510280

出　　处：《心脏杂志》2010年第6期807-810,共4页Chinese Heart Journal

基　　金：中国博士后科学基金资助(20080440215);广东省自然科学基金资助(2157000068;8151051501000062);广东省和广州市科技计划项目(2009B11000018;2009JI-c491)

摘　　要：目的:探讨可诱导共刺激分子(ICOS)在人外周血T细胞的表达及氧化低密度脂蛋白(ox-LDL)的干预作用。方法:以人外周血T细胞为实验模型,通过间接免疫荧光法、RT-PCR和Western blot等方法,观察ox-LDL对人外周血T细胞表达ICOS的影响。结果:①ICOS表达于人外周血T细胞膜,荧光信号呈点状散在分布于细胞表面。RT-PCR检测显示,ICOS mRNA的扩增片段位于相当于Marker 368 bp的位置。Western blot检测显示,ICOS的分子量约为55 kD。②ox-LDL刺激组ICOS的吸光度(A)值高于空白对照组,提示ox-LDL能够明显增加人外周血T细胞中的ICOS mRNA和其蛋白的表达(P<0.05)。结论:ICOS表达于人外周血T细胞表面,ox-LDL能够上调ICOSmRNA和其蛋白的表达,这可能是动脉粥样硬化的免疫学发病机制之一。AIM： To study the expression of inducible costimulator （ICOS） on human peripheral blood T ceils （HPBTC） and the intervening effect of oxidized low-density lipoprotein （ox-LDL）. METHODS： HPBTCs were cultured in vitro and incubated with 100 mg/L ox-LDL for 24 h. ICOS expression levels were determined by fluorescence, reverse transcription PCR （RT-PCR） and Western blotting, respectively. RESULTS： Fluorescence microscopy showed that fluorescence signals of positive cells expressed by ICOS were distributed on cell membrane in a spot-style. RT-PCR of mRNA derived from the cultured HPBTC showed products of the expected size （368 bp）. Western blotting of protein verified HPBTC expression of ICOS as a cell-associated protein with a molecular mass of 55 kDa. ox-LDL significantly increased in the expression of ICOS. Similar effects were seen in both expressions of mRNA and protein （P 〈 0. 05 ）. CONCLUSION： ICOS can express on HPBTC and ox-LDL upregulates the expression of ICOS, which may be one of the immunomechanisms of atherosclerosis.

关 键 词：可诱导共刺激分子 氧化低密度脂蛋白 人外周血T细胞 动脉粥样硬化 

分 类 号：R392.12[医药卫生—免疫学]