微囊化异种嗅球组织移植联合药物干预修复受损脊髓的研究  

Combining exnotransplantation of microencapsulated olfactory bulb and drug intervention to repair the injured spinal cord

作　　者：王航辉[1] 朱文侠[1] 李小记[1] 韩振奎[1] 王璐[1] 赵琳[1] 刘伯锋[1] 

机构地区：[1]延安大学医学院解剖学教研室,延安716000

出　　处：《神经解剖学杂志》2010年第6期620-624,共5页Chinese Journal of Neuroanatomy

基　　金：陕西省自然科学基金(08JK502);延安市科技计划项目(2009KS-22);延安大学专项科研基金(YD2008-39)

摘　　要：目的:研究微囊化异种嗅球组织移植联合β-七叶皂甙钠对脊髓继发性损伤的治疗保护作用及机制。方法:健康SD大鼠180只,随机分为损伤对照组、微囊化兔嗅球组织移植组、微囊化兔嗅球组织移植联合β-七叶皂甙钠治疗组,损伤组又设1、3、7、14、28d5个时相点,紫外分光光度计检测脊髓伊文氏蓝含量;免疫组织化学观察血管内皮生长因子(VEGF)的表达;神经功能评分法(BBB法)评价大鼠术后运动功能的恢复情况。结果:脊髓损伤后脊髓伊文氏蓝含量增加、VEGF表达下调,大鼠的运动功能缺失,经微囊化异种嗅球组织移植联合β-七叶皂甙钠治疗后血脊髓屏障的通透性有所改善,VEGF表达上调,大鼠的运动功能有所恢复。结论:微囊化异种嗅球组织移植对脊髓继发性损伤有较好的疗效,联合应用嗅球组织细胞和β-七叶皂甙钠在脊髓损伤修复治疗中具有协同作用。Objective：To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium in rats at the early stages of spinal cord injury.Methods：Healthy 180 SD rats were divided into three groups as following：injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium group,the later two groups were quantified at 1,3,7,14,and 28 d after spinal cord injury.Evans Blue content in the spinal cord was examined with the ultraviolet spectrophotometer;the change of vascular endothelial growth factor（VEGF） expression after spinal cord injury was detected by immunohistochemical method.BBB score was designed to assess its behavioral ability.Results：Evans Blue content was increased and expression of VEGF were significantly decreased,loss of motor function in rats after spinal cord injury was observed.Permeability of blood-spinal barrier was improved,VEGF was obviously upregulated,and motor function was recovered in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium.Conclusion：Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in certain extent.There may be a synergistic effect between olfactory ensheating cells and β-aescinate sodium.

关 键 词：脊髓损伤 异种移植 嗅鞘细胞 血管内皮生长因子 Β-七叶皂甙钠 大鼠 

分 类 号：R651.2[医药卫生—外科学]