MBL抑制Jurkat细胞增殖和分泌IL-2  

Mannan-binding lectin inhibits proliferation of Jurkat cells and secretion of IL-2

作　　者：王明永[1] 张雅妮[1] 雷鸣[1] 张丽芸[1] 卢晓[1] 陈政良[1] 

机构地区：[1]南方医科大学免疫学教研室,广州510515

出　　处：《现代免疫学》2010年第6期448-452,共5页Current Immunology

基　　金：国家自然科学基金资助项目(30972679)

摘　　要：甘露聚糖结合凝集素（MBL）在天然免疫中起关键作用,但其对T淋巴细胞介导获得性免疫应答是否有影响尚不得而知。用非特异性刺激物PHA、PMA及ionomycin活化人T淋巴细胞株Jurkat细胞,ELISA和流式细胞术检测MBL与Jur-kat细胞的结合能力,WST-1和ELISA分析MBL对PHA、PMA及ionomycin活化Jurkat细胞的增殖及分泌细胞因子IL-2的影响。结果显示：MBL可结合PHA/PMA活化的Jurkat细胞,与未活化Jurkat细胞结合微弱;高浓度MBL（10~20mg/L）能以浓度依赖的方式抑制PHA/PMA活化的Jurkat细胞增殖,加入外源IL-2不能逆转这种作用;高浓度MBL（10~20 mg/L）能抑制PMA/ionomycin活化的Jurkat细胞分泌细胞因子IL-2,且呈剂量依赖关系。提示MBL可能在调节T细胞介导的获得性免疫应答中起一定作用。Much less information is known about the role of mannan-binding lectin（MBL） in the regulation of T cell-mediated acquired immune responses mediated by T cells,although it is believed to be of importance in innate immunity.In the present study,T lymphoid cell line Jurkat cells were activated by non-specific stimulatants,such as phytohemagglutinin（PHA）,PMA or ionomycin,and the binding of MBL to Jurkat cells was measured by ELISA and FACS.Meanwhile,the impact of MBL on the proliferation of Jurkat cells activation by PHA,PMA or ionomycin and the secretion of IL-2 was analyzed by ELISA and WST-1 assay.It was shown that MBL could significantly bind to PHA/PMA-activated Jurkat cells but slightly bind to the un-activated cells.Also,MBL could significantly inhibit the proliferation of PHA/PMA-activated Jurkat cells at higher concentrations（10~20 mg/L） in a dose-dependent manner,which was not altered by addition of rhIL-2;and that MBL could inhibit IL-2 secretion by PMA/ionomycin-activated Jurkat cells at higher concentrations（10~20 mg/L） in a concentration-dependent manner.All these data suggest that MBL may perform some functions in T cell-mediated acquired immune responses mediated by T cells.

关 键 词：甘露聚糖结合凝集素 JURKAT细胞 T淋巴细胞 免疫调节 

分 类 号：R392[医药卫生—免疫学]