Tim-3在CD8^+ T细胞上的表达及功能研究  

Expression of T cell immoglobulin mucoprotein 3 on CD8^+ T cells and its functional study

作　　者：汪峰[1] 孙自镛[1] 朱旭慧[1] 廖亚龙[1] 汪月[1] 刘彩林[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《现代免疫学》2010年第6期458-461,共4页Current Immunology

摘　　要：为探讨CD8+T细胞是否表达T细胞免疫球蛋白黏蛋白3(Ti m-3),并探讨Ti m-3分子是否和CD8+T细胞功能相关,通过致敏C57BL/6小鼠(取BALB/c小鼠皮肤移植到C57BL/6小鼠背部),术后第7天处死小鼠,分离小鼠引流淋巴结,流式细胞术观察CD8细胞上CD44、CD62L、Ti m-3表达;术后第10天分离受者脾细胞,流式分选CD8+T细胞,以此作为效应细胞去杀伤BALB/c小鼠脾细胞,实验组和对照组分别加入Ti m-3封闭抗体和PBS。结果发现,Ti m-3表达于活化的CD8+T细胞(表型为CD8+CD44highCD62Llow),但不表达于初始的CD8+T细胞。此外,使用Ti m-3封闭抗体后可减弱细胞毒性CD8+T细胞引起的杀伤反应,且在体内减少IFN-γ分泌水平。结果表明,Ti m-3分子表达于活化的CD8+T细胞,且和CD8+T功能相关。To determine whether T cell immunoglobulin 3（Tim-3） is expressed on CD8^＋ T cells,and to investigate its function on CD8^＋ T cells,C57BL/6 mice were transplanted on the back with skin grafts taken from BALB/c mice.Cells from the draining lymph node of the skin sensitized recipients were harvested on day 7 after transplantation and the expressions of CD8,CD44,CD62L and Tim-3 on these cells were analyzed.Splenocytes from recipients were harvested on day 10 after transplantation and CD8^＋ T cells were purified by sorting CD8^＋ populations via FACSAria.The purified CD8^＋ T cells were used as effector cell to kill splenocytes of BALB/c mice.Either Tim-3 blocking antibody or PBS was added to culture medium in the experimental group and control group of mice.Our results indicated that Tim-3 was expressed on activated CD8^＋ T cells（CD8^＋CD44highCD62Llow）,but not on nave CD8^＋ T cells.Furthermore,Tim-3 pathway was also involved with the function of CD8^＋ T cells.Administration of Tim-3 blocking antibody obviously decreased CD8^＋ alloreactive T cells mediating cytotoxicity and reduced the secretory level of cytokine IFN-γ.It is evident Tim-3 molecules are expressed on activated CD8^＋ T cells and also involved with the function of these cells.

关 键 词：TIM-3 CD8^+T细胞 细胞毒性 IFN-Γ 

分 类 号：R392.4[医药卫生—免疫学]