双价抗虫转基因棉花研究  

Development of Bivalent Insectresistant Transgenic Cotton Plants

作　　者：郭三堆[1] 崔洪志[1] 夏兰芹[1] 武东亮[1] 倪万潮[2] 张震林[2] 张保龙[2] 徐英俊[2] 

机构地区：[1]中国农业科学院生物技术研究中心 [2]江苏省农业科学院经济作物研究所

出　　处：《中国农业科学》1999年第3期1-7,共7页Scientia Agricultura Sinica

基　　金：国家"863"计划;农业部棉花专项基金;中华农业科教基金

摘　　要：构建了携带人工合成的ＧＦＭＣｒｙＩＡ杀虫基因和经过修饰的ＣｐＴＩ基因的高效双价杀虫基因植物表达载体ｐＧＢＩ１２１Ｓ４ＡＢＣ。采用花粉管通道法，将ｐＧＢＩ１２１Ｓ４ＡＢＣ转入到石远３２１、中棉所１９号、３５１７和５４１中国棉花生产品种中，首次获得了双价转基因抗虫棉株系。叶片室内抗虫生物学鉴定表明，抗性好的株系棉铃虫幼虫校正死亡率大于９６％；经分子检测，证实了双价杀虫基因在棉花基因组中的整合与表达。By pollen tube pathway method, the plant expression vector pGBI121S4ABC, harboring both synthesized Bt insecticidal protein gene and modified CpTI gene, was introduced into the elite cotton(Gossypium hirsutum L. cultivars Shiyuan 321, Zhongmiansuo 19, 3517 and 541. Resistance of the leaves of these transgenic plants to cotton boll worm was identified. The results showed that some cotton plants were highly toxic to the insect with larvae death rate up to 96%. The results of PCR, PCRsouthern and RTPCR analyses confirmed the integration and expression of the two gene in these insectresistant cotton plants.

关 键 词：棉花 双价 抗虫转基因棉花 CPTI BT杀虫基因 

分 类 号：S562.03[农业科学—作物学]