玉米轮回选择群体遗传多样性RAPD分子标记评估  

Genetic Diversity Evaluation of Maize Recurrent Selection Population With RAPD Marker

作　　者：刘勋甲[1] 郑用琏[1] 刘纪麟 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室

出　　处：《中国农业科学》1999年第3期14-20,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金

摘　　要：采用ＲＡＰＤ标记技术，评估中国西南地方优良种质巫溪（Ｗ）、美国玉米带坚秆综合种ＢＳＳＳ９（Ｂ）、墨西哥热带种质墨黄九（Ｍ）及以它们为主体亲本人工合成的ＷＢＭＣ０基础群体、改良一轮的ＷＢＭＣ１群体的遗传变异。结果表明：（１）１５个１０－ｍｅｒ随机引物共扩增８９个ＤＮＡ片段，其中显示多态性的片段，在Ｗ、Ｂ、Ｍ、ＷＢＭＣ０、ＷＢＭＣ１中分别为６８、６７、７１、７６和８２个，其多态片段比例依次为７６．４％、７５．３％、７８．９％、８５．４％和９２．１％；（２）５个群体各８９个片段的平均杂合度Ｗ为０．２８５、Ｂ为０．２５２、Ｍ为０．２９６、ＷＢＭＣ０为０．３２７、ＷＢＭＣ１为０．３４６，以ＷＢＭＣ０和ＷＢＭＣ１较大；（３）５个群体各８９个片段的平均遗传距离Ｗ为０．２５３３、Ｂ为０．２２４６、Ｍ为０．２４８１、ＷＢＭＣ０为０．３００６、ＷＢＭＣ１为０．３１１９，也以ＷＢＭＣ０和ＷＢＭＣ１较大；（４）累加各引物扩增的基因型种类，Ｗ为１３６、Ｂ为１１７、Ｍ为１２８、ＷＢＭＣ０为１５１、ＷＢＭＣ１为１５０。以上结论说明，人工合成的广基玉米群体，遗传多样性比亲本群体丰富，且这种多样性属ＤＮＡ变异，并非环境造成。经过一轮选择后的?The genetic diversity of a maize population Wuxi(W) from Southwest China, BSSS9 (B) from America, Mohuangjiu (M) from Mexico , WBMC0 synthesized with W, B, M as main parents, WBMC1 one cycle selected from WBMC0 was evaluated by RAPD molecular marker.The results showed that (1)totally 89 fragments(loci)were amplified by 15 10mer random primers,the proportion of polymorphic loci were W 76.4%, B 75.3%, M 79.8%, WBMC0 85.4% and WBMC1 92.1% respectively; (2)the mean gene heterozygosity based on 89 loci was W 0.285, B 0.252, M 0.296,WBMC0 0.327 and WBMC1 0.346;(3)the mean genetic distances based on 89 loci were W 0.2533, B 0.2246, M 0.2481, WBMC0 0.3006, WBMC1 0.3119;(4) the genotypic numbers accumulated by 15 primer amplification were W 136,B 117,M 128,WBMC0 151 and WBMC1 150.All indexes indicated that the synthesized maize populations were more polymorphic than the parent populations in DNA level.One cycle selection did not reduce the variation. The principle and technical system were discussed for evaluating genetic variation of reccurent selection population using RAPD molecular marker.

关 键 词：玉米 遗传多样性 RAPD评估 分子标记 轮回选择 

分 类 号：S513.032[农业科学—作物学]