快生型大豆根瘤菌血清学特性的研究  

Study on the Serological Characteristics of Sinorhizobium fredii

作　　者：李镇[1,2] 戴经元[1,2] 周俊初[1,2] 

机构地区：[1]华中农业大学 [2]农业部农业微生物重点实验室

出　　处：《中国农业科学》1999年第3期27-32,共6页Scientia Agricultura Sinica

基　　金：欧盟合作研究STD3-CT92-0127资助课题

摘　　要：以自新疆、黑龙江、辽宁、吉林、湖北、天津和北京等地分离的１６株快生型大豆根瘤菌为供试菌，用交叉凝集反应比较研究了它们与目前国内外已报道的９株快生型大豆根瘤菌标准血清型菌株之间的血清学关系，研究结果表明：（１）菌株Ｊ７１１，ＮＪ１６，ＮＪ１１１和Ａ２１２均属Ｏ１（２０４８）血清型。（２）菌株ＲＴ１５、Ｔｗｏ－７和９３Ｆ４１与供试标准血清型菌株的菌体抗原均不相同，命名为Ｏ９、Ｏ１０和Ｏ１１３种新的血清型。菌株６－１为自凝菌株，其血清型为Ｏ０。（３）交叉抗原吸收试验结果表明，菌株９２Ｘ１０、Ｃ３３３、ＲＴ１０和２０５６同属Ｏ５血清型，并可进一步区分为Ｏ５ａｂ、Ｏ５ｂｃｄ、Ｏ５ｄｅｆ和Ｏ５ｆｇ４个血清亚型。菌株ＨＡ１０－１－３、ＨＡ１２－１－１２和２１２０分属Ｏ６血清型的Ｏ６ａｂ、Ｏ６ｂｃｄ和Ｏ６ｄｅ３个血清亚型。（４）将２０４８等６个标准菌株重新命名为Ｏ１，Ｏ２，Ｏ３，Ｏ４，Ｏ７和Ｏ８血清型。The serological relationship between 16 strains isolated from different areas in China and 9 standard serotype strains of S.fredii were compared by the crossagglutination reaction method.It was found that RT15,Two7 and 93F41 showed no reactions with standard serotype strains tested and therefore, were classified as new serotypes O9,O10 and O11 respectively. The strain 61 was classified as O0 serotype because of its autoagglutination.Strains J711,NJ16, A212 and NJ111 were identified as O1(2048) serotype.The results of antigen crossabsorption tests revealed that subserotypes existed between serotypes of S.fredii. Independent standard serotype strains C333, 2056,RT10 and 92X10 were classified as O5 serotype, and O5ab,O5bcd,O5def and O5fg subserotypes;on further step, HA1013,HA12112 and 2120 were classified as subserotypes O6ab,O6bcd and O6de of the serotype O6; Moreover, 2048 and other five standard serotype strains were renamed as O1,O2,O3,O4,O7 and O8 serotypes.

关 键 词：快生型 大豆根瘤菌 血清型 交叉凝集反应 

分 类 号：S154.381[农业科学—土壤学] Q939.114[农业科学—农业基础科学]