PEG渗调对苦瓜种子活力和膜脂过氧化的影响  

The Effect of Osmoconditioning with PEG on the Vigor of Balsampear (Momordica charantia L.) Seeds and Peroxidation of Cell Membrance Lipids

作　　者：黄祥富[1] 蒋明兰 廖军 

机构地区：[1]中山大学生命科学学院,广州510275 [2]西江大学生物系,肇庆526060 [3]深圳光明中学,深圳518107

出　　处：《种子》1999年第2期7-9,共3页Seed

摘　　要：15％～35％PEG能提高苦瓜种子活力，但以25％PEG渗调效果最好，经25％PEG渗调4小时，发芽率、生长势和活力指数分别由对照的62．5、3．05和1．95提高到90．6、7．88和7．15。实验表明，PEG可明显提高苦瓜种子SOD和CAT的活性，并能抑制LOX的活性。经25％PEG处理后SOD和CAT的活性分别由对照的620、7，2提高到111．8和11．4，LOX活性由1．27降低到0．95。PEG渗调对恢复种子膜的完整性是明显的，25％PEG处理后，电导率由对照的143.5下降到79．4。The vigor of balsampear seeds could be raised by osmoconditioning with 15 % - 35 % concentration PEG, and the effect of 25% PEG was best. After soaking for 4 hours in 25% PEG solution, germination percentage, germination index and vigor index were in creased from 62. 5,5. 27, 1. 95 to 90. 6, 7. 3 and 7. 15 respectively. The result showed the activities of SOD and CAT were increased by osmoconditioning with PEG, and the activity of LOX was declined. After osmoconditioning with 25% PEG, the permeability of ecll membrance was improved and electral conductivity of the solution decreased.

关 键 词：苦瓜 种子 渗透调节 种子活力 膜脂过氧化 PEG 

分 类 号：S642.502[农业科学—蔬菜学] S330.31[农业科学—园艺学]