天府肉鹅IFN-α基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of IFN-α gene of Tianfu meat-type goose

作　　者：刘菲[1,2] 赵婷婷[1] 程安春[1,2] 汪铭书[1,2] 韩清林[1,3] 高丽芹[1] 唐卫杰 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014 [3]四川省大英县畜牧局,四川大英629118

出　　处：《中国兽医科学》2010年第12期1271-1275,共5页Chinese Veterinary Science

基　　金：四川省教育厅自然科学基金重点项目(2006A012);教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848);现代农业产业技术体系建设专项项目(nycytx-45-12)

摘　　要：采用RT-PCR方法从天府肉鹅外周血单核细胞中扩增了IFN-α基因;并将上述扩增片段克隆到pMD18-T载体中,构建了重组质粒。用生物信息学软件及在线分析方法对IFN-α基因的核苷酸序列及其推导的氨基酸序列进行了分析,同时与13个物种的IFN-α基因进行了同源性比较。结果表明,成功地克隆了IFN-α基因,该基因与同类水禽(鸭、鹅)的IFN-α基因有90%以上的同源性,在进化树中位于同一分支,研究结果为开发鹅的干扰素制剂奠定了基础。An IFN-α gene was amplified from the peripheral blood mononuclear cells of Tianfu meat-type goose using RT-PCR and was cloned into the vector pMD18-T to construct the recombinant plasmid.The nucleotide sequence of the amplified IFN-α gene and the amino acid sequence deduced from the gene were then analyzed by the bioinformatics software and on-line tools.The similarity and evolution of IFN-α gene were analyzed among Tianfu meat-type goose and next 13 species.These results revealed that the cloned-successfully IFN-α gene shared over 90% similarity with that of the 13 species of waterfowls（goose and duck）,and they were at the same branch in the phylogenetic tree.The study laid the foundations for development of goose interferon reagent.

关 键 词：干扰素 克隆 生物信息学 

分 类 号：Q511[生物学—生物化学] Q785