慢性心力衰竭大鼠心房血管紧张素Ⅱ1型受体和尼古丁α7受体的表达  

Expression of atrial angiotensin Ⅱ type 1 receptor and α7-nicotinic acetylcholine receptor during development of heart failure in rats

作　　者：杨中伟[1] 赵婷[1] 李冬洁[1] 倪敏[1] 沈甫明[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2010年第12期1282-1285,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30870940);上海市基础研究重点项目(08JC1406000)~~

摘　　要：目的观察血管紧张素Ⅱ1型受体(AT1R)和尼古丁α7受体(α7nAChR)在心力衰竭大鼠心房的表达。方法将雄性SD大鼠随机分成4组,行腹主动脉缩窄术,制备心衰模型。采用蛋白质印迹法,比较缩窄4、8、12和16周后对照组和缩窄组大鼠心房AT1R和α7nAChR的表达变化情况。结果与同时间点对照组相比,心房AT1R和α7nAChR的表达在缩窄后4、8、12和16周差异均无统计学意义。结论在腹主动脉缩窄致大鼠心衰模型中,心房AT1R和α7nAChR可能并不参与心衰早期发生、发展的病理过程。Objective To observe the atrial expression of angiotensin Ⅱ receptor type 1（AT1R） and α7-nicotinic acetylcholine receptor（α7nAChR）during the development of heart failure in rats.Methods Male SD rats were randomly divided into 4 groups.Abdominal aorta coarctation （AAC） was used to prepare heart failure model.Expression of AT1R and α7nAChR was determined by Western blotting analysis at 4,8,12,and 16 weeks after AAC in all the groups.Results Compared with the control group,expression of AT1R and α7nAChR in AAC group was not significantly different at all the 4 time points after AAC.Conclusion Our findings suggest that atrial AT1R and α7nAChR may not be involved in the early stage pathological process of AAC-induced heart failure.

关 键 词：心力衰竭 心房 1型血管紧张素受体 尼古丁α7受体 

分 类 号：R541.6[医药卫生—心血管疾病]