HCV HVR1模拟肽诱导小鼠树突状细胞免疫应答及机制的初步研究  

Preliminary study of immune response of DC in mice induced by a mimic peptide from HCV HVR1

作　　者：杨斌[1] 宋春辉[1] 迟淑萍[1] 陈黎明[1] 程云[1] 

机构地区：[1]解放军第三○二医院肝病生物治疗研究中心,北京100039

出　　处：《胃肠病学和肝病学杂志》2010年第12期1092-1094,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家863基金资助(2006A02Z494);国家重大创制新药(2009zx09102-001)

摘　　要：目的探讨HCV HVR1模拟肽(7肽)诱导小鼠树突状细胞免疫应答模式及其机制。方法用"Bu lk培养法"制备树突状细胞,经7肽免疫10天的小鼠,取脾细胞,分选CD8+T细胞与经过7肽负载并成熟树突状细胞共孵育24 h,收集上清,应用流式细胞技术方法检测上清细胞因子。结果此方法培养的细胞表面CD11 c的表达率超过80%,具有典型的DC形态;实验组中CD8+T上清中IFNγ-、TNF-α、IL-10及IL-6较对照组有所升高,均具有统计学意义。结论利用此法能在体外培养出大量的未成熟DC;7肽能诱导以CD8+T细胞为主的细胞免疫。Objective To investigate the immune response and mechanism of dendritic cells（DC） induced by a polypeptide（7P）. Methods The method of ＂Bulk＂ was used to harvest DCs.After subcutaneously immunization for ten days,the mouse splenocytes were harvested.CD8＋T cells were separated from splenocytes by cell sorting technology.Then CD8＋T cells and DC cells were incubated for 24 h.The supernatants were detected by cytometric bead array protein array system（CBA）. Results The expression rate of CD11c＋ cultured in vitro was more than 80%.Compared with control group,the levels of IFN-γ,TNF-α,IL-10 and IL-6 secreted from CD8＋T cell were increased in experiment group,and the difference was statistically significant between the two groups. Conclusion A large number of DCs from mouse bone marrow in vitro exhibited typical morphology of DC.The peptide could induce cell-mediated immune response through CD8＋T cells.

关 键 词：丙肝病毒 树突状细胞 模拟肽 免疫应答 

分 类 号：R512.6[医药卫生—内科学]