电针对链脲佐菌素AD模型大鼠海马区Aβ阳性细胞表达和脑内SOD活性的影响  

Effects of electroacupuncture on expression of Aβ positive cells of the hippocampus and SOD activity in rats with streptozocin-Alzheimer's disease

作　　者：张鹏[1] 关珊珊[1] 姜国华[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国针灸》2010年第12期1007-1010,共4页Chinese Acupuncture & Moxibustion

基　　金：黑龙江省教育厅理科面上项目:11531339

摘　　要：目的:探讨电针对阿尔茨海默病(AD)大鼠学习记忆能力改善的可能机制。方法:将雄性Wistar大鼠60只随机分成正常组、盐水组、模型组、西药组和电针组,每组12只。除正常组、盐水组外,其余3组侧脑室注射链脲佐菌素(STZ)制备动物模型,盐水组以同等剂量注射生理盐水。西药组给予美金刚灌胃治疗;电针组电针"百会""大椎""太溪""肾俞""足三里",每日1次,7日为一疗程,共治疗4个疗程;其他3组常规饲养,不予干预。以Morris水迷宫评价大鼠学习记忆能力,用免疫组化法和可见光分光光度计比色法观测大鼠海马区Aβ阳性细胞表达情况和脑内超氧化物歧化酶(SOD)活性情况。结果:与正常组及盐水组比较,模型组海马区Aβ蛋白表达水平升高(均P<0.01),SOD活性降低(均P<0.01)。治疗后电针组、西药组与模型组比较,Aβ蛋白表达水平降低(均P<0.01),SOD活性升高(均P<0.01)。结论:电针可能通过降低Aβ蛋白表达,提高SOD活性来改善AD大鼠学习记忆能力。Objective To investigate the possible mechanism of the electroacupuncture for improvement of learning and memory in rats of Alzheimer＇s disease （AD）. Methods Sixty Wistar rats were randomly divided into a normal group, a normal saline group, a model group, a western medication group and an electroacupuncture group, 12 rats in each group. The AD rat model was established by injecting Streptozocin （STZ） into lateral cerebral ventricle, except the rats in the normal saline group injecting Normal Saline with the same dose and in normal group with no injection. The western medication group was treated with intragastric administration of Memantine, and in the electroacupuncture group, the electroacupuncture was given at ＂Baihui＂ （GV 20）, ＂Dazhui＂ （GV 14）, ＂Taixi＂ （KI 3）, ＂Shenshu＂ （BL 23）, ＂Zusanli＂ （ST 36）, once each day, 7 days for a course, and lasted for 4 courses. The other three groups were fed in routine way and without any treatment. The learning and memory ability was assessed by Morris water maze and the expression of Aβ positive cells of the hippocampus and superoxide dismutase （SOD） activity were determined by immunohistochemistry and visible spectrophotometer colorimetry. Results Compared with the normal group and the normal saline group, the Aβ protein expression was significantly increased in the model group （both P〈0.01）, and the SOD activity was obviously decreased （both P〈0.01）. After treatment, as compared with the model group, the Aβ protein expression was significantly decreased （both P〈0.01）, and the SOD activity was obviously increased （both P〈0.01） in the electroacupuncture group and western medication group. Conclusion Electroacupuncture treatment can reduce the Aβ protein expression and increase the SOD activity of the hippocampus so as to .improve learning and memory ability in the AD rats.

关 键 词：电针 阿尔茨海默病 AΒ蛋白 超氧化物歧化酶 

分 类 号：R246.6[医药卫生—针灸推拿学]