阻断缝隙连接对膀胱经腧穴组织氧分压的影响  

Effects of gap junction blocking on the oxygen partial pressure in acupoints of the Bladder Meridian

作　　者：王琪[1] 余炜昶[1] 蒋红芝[1] 陈胜利[2] 张明敏[3] 孔鄂生[4] 黄光英[1] 

机构地区：[1]华中科技大学附属同济医院中西医结合研究所,湖北武汉430030 [2]华中科技大学同济医学院药物分析学系,湖北武汉430030 [3]华中科技大学附属同济医院中西医结合科,湖北武汉430030 [4]华中科技大学同济医学院环境医学研究所,湖北武汉430030

出　　处：《中国针灸》2010年第12期1011-1014,共4页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助重点项目:90209009

摘　　要：目的:探讨缝隙连接与经络现象之间的联系。方法:分别采用1-庚醇阻断山羊腧穴缝隙连接及小鼠缝隙连接蛋白43(Cx43)基因敲除方法,用新型组织氧分压传感针检测山羊及小鼠膀胱经经穴("肝俞""大肠俞"等)及旁开非经穴处的氧分压。结果:①山羊膀胱经经穴的组织氧分压高于其旁开对照点,两者间差异有统计学意义(P<0.01);②注射1-庚醇后,山羊膀胱经经穴的氧分压较未注射前及注射生理盐水均明显升高(均P<0.01);③缝隙连接蛋白Cx43野生型(WT)小鼠膀胱经经穴的组织氧分压明显高于其旁开非经穴点,差异有统计学意义(均P<0.01);缝隙连接蛋白Cx43杂合型(HT)小鼠上述各穴位点的组织氧分压也高于其旁开对照点,但差异无统计学意义(均P>0.05);④WT小鼠各穴位点的组织氧分压均明显高于HT小鼠(均P<0.05),而各非经穴点的氧分压两组间比较差异无统计学意义(均P>0.05)。结论:缝隙连接在针刺信号转导过程中具有重要作用。Objective To explore the relation between gap junction and meridian phenomenon. Methods The oxygen partial pressure in acupoints [＂肝俞＂（BL 18）＂大肠俞＂（BL 25） etc. ] and in their corresponding non-acupoints of the Bladder Meridian was observed with the needle-type tissue oxygen tension sensor in the gap junction blocking goats by 1-Heptanol injection and the Connexin 43 （Cx43） gene knockout mice. Results① The oxygen partial pressure in acupoints of Bladder Meridian on goats was higher than that in non-acupoints after 1-Heptanol injection with significant differences between them （ both P〈0.01）. ② The oxygen partial pressure in acupoints of Bladder Meridian on goats increased significantly after injecting 1-Heptanol as compare with that either injecting normal saline or injecting nothing with significant differences between them （all P〈0.01）. ③ The oxygen partial pressure in acupoints of the Bladder Meridian was significantly higher than that in the non-acupoint controls in Cx43 wild type （WT） mice （all P〈0.01）. In Cx43 heterozygote （HT） mice, the oxygen partial pressure between acupoints and non-acupoint controls showed no significant differences （all P〉0.05）. ④ In acupoints, the oxygen partial pressure in Cx43 WT mice was significantly higher than that in Cx43 HT mice （all P〈0.05）, while in the corresponding non-acupoints, this difference had no statistically significant （all P〉0.05）. Conclusion Gap junction maybe the essential factor in signal transduction of acupuncture.

关 键 词：血气监测 经皮 传感针 连接蛋白43 经络生理 膀胱经 

分 类 号：R245.2[医药卫生—针灸推拿学]