裸花紫珠的化学成分及其止血活性研究  

Chemical constituents from Callicarpa nudiflora and their hemostatic acitivity

作　　者：张洁[1] 李宝泉[1] 冯锋[1] 唐于平[2] 柳文媛[3] 

机构地区：[1]中国药科大学天然药物化学教研室,江苏南京210009 [2]南京中医药大学江苏省方剂研究重点实验室,江苏南京210046 [3]中国药科大学药物分析教研室,江苏南京210009

出　　处：《中国中药杂志》2010年第24期3297-3301,共5页China Journal of Chinese Materia Medica

摘　　要：目的:研究马鞭草科紫珠属植物裸花紫珠Callicarpa nudiflora的化学成分,并筛选其止血活性成分。方法:应用硅胶及Sephadex LH-20柱色谱方法分离纯化,运用波谱数据解析鉴定化合物结构;测定分离得到的化合物对家兔体外血液凝集参数凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)及纤维蛋白原(FIB)含量的影响。结果:分离鉴定了11个化合物,其中2个二萜:7α-hydroxy sandaracopimaric acid(1),16,17-dihydroxy-3-oxophyllocladane(2);2个酚苷:acte-oside(3),samioside(4);3个三萜:2α,3α,24-三羟基-乌索烷-12-烯-28-酸(5),2α,3α,19α-三羟基-乌索烷-12-烯-28-O-β-D-葡萄糖苷(6),2α,3α,19α,23-四羟基-乌索烷-12-烯-28-O-β-D-葡萄糖苷(7);4个黄酮:鼠李秦素(8),5-羟基-3,7,4′-三甲氧基黄酮(9),5-羟基-3,7,3′,4′-四甲氧基黄酮(10),木犀草苷(11)。与空白对照组相比,所有化合物均无明显缩短PT的作用(P<0.01),化合物3,4,7,10有显著缩短APTT的作用(P<0.01),化合物5对TT有明显的延长作用(P<0.01),化合物8对FIB有显著增加的作用(P<0.01)。结论:化合物2,4,10为首次从紫珠属植物中分离得到,化合物1,3,5,6,7,9为从裸花紫珠中首次分得。裸花紫珠可能是通过影响内源性凝血途径发挥止血作用。Objective： To study the hemostatic effect of chemical constituents from Callicarpa nudiflora.Method： The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography.Their structures were determined on the basis of spectral analysis.prothrombin time（PT）,activated partial thromboplastin time（APTT）,thrombin time（TT）and fibrinogen（FIB）of the constituents rabbit blood samples were tested with rabbit blood in vitro.Result： Eleven compounds were isolated and identified as two diterpenens：7α-hydroxy sandaracopimaric acid（1）,16,17-dihydroxy-3-oxophyllocladane（2）.Two phenoic glycosides：acteoside（3）,samioside（4）.Three triterpenes：2α,3α,24-trihydroxy-ursa-12-en-28-oic acid（5）,2α,3α,19α-trihydroxy-ursa-12-en-28-oic acid-28-O-β-D-glucopyranosyl ester（6）,and 2α,3α,19α,23-tetrahydroxy-ursa-12-en-28-oic acid-28-O-β-D-glucopyranosyl ester（7）.Four flavones： rhamnazin（8）,5-Hydroxy-3,7,4′-trimethoxy-flavone（9）,5-Hydroxy-3,7,3′,4′-tetramethoxyflavone（10）,and luteoloside（11）.All Compounds can not significantly shorten the PT（P〈0.01）,compounds 3,4,7,10 can remarkedly increase APTT（P〈0.01）,compound 5 can prolong the TT（P〈0.01）obviously,and compound 8 can significantly increase the contents of FIB（P〈0.01）.Conclusion： Compounds 2,4 and 10 were isolated from this genus for the first time,and compounds 1,3,5,6,7 and 9 had been isolated from this plant for the first time.The hemostatic effect of C.nudiflora may be related to the activation of the intrinsic blood coagulation system.

关 键 词：裸花紫珠 化学成分 止血活性 体外 

分 类 号：R284.1[医药卫生—中药学]