近红外漫反射法测定杜仲中松脂醇二葡萄糖苷的含量  

Quantitative determination of pinoresinol diglucoside in Eucommiae unloads by NIRS

作　　者：李伟[1] 孙素琴[2] 覃洁萍[3] 易艳红[1,4] 杨美华[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100193 [2]清华大学化学系,北京100084 [3]广西中医学院,广西南宁53001 [4]北京中医药大学,河北廊坊065001

出　　处：《中国中药杂志》2010年第24期3318-3321,共4页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2006BAI09B05)

摘　　要：目的：用近红外光谱技术快速测定杜仲中松脂醇二葡萄糖苷的含量。方法：选取了3个不同产地的41个杜仲样品,用高效液相色谱法测定其松脂醇二葡萄糖苷含量,用近红外光谱仪漫反射方式在12 0004 000 cm-1采集相应样品的光谱,利用仪器自带的OPUS软件优选了光谱的预处理方法,并用偏最小二乘法（PLS）建立松脂醇二葡萄糖苷含量和光谱数据之间的相关性模型。结果：对光谱进行一阶导数和减去一条直线相结合处理后,在7 5024 597.6 cm-1能最有效地提取光谱中的信息,经交叉验证后校正集相关系数（R2）为0.926 4,校正集标准偏差（SEC）为0.029,预测集标准偏差（SEP）为0.026。结论：杜仲中松脂醇二葡萄糖苷含量和近红外光谱之间存在良好的相关性,适合于此中药指标性成分的快速分析。Objective： To establish a rapid determination method of pinoresinol diglucoside in Eucommiae unloads by near-infrared reflectance spectroscopy（NIRS）.Method： Forty-one samples of E.unloads were collected from three different producing areas and their main component,namely pinoresinol diglucoside,was determined by HPLC.Corresponding data of samples were collected from 12 000 to 4 000 cm-1 by near-infrared reflectance spectroscopy.The spectral pretreatment was optimized by OPUS software and the calibration equations between the content of pinoresinol diglucoside and spectrum data were constructed by partial least squares regression.Result： Available information could be extracted from spectra in the range from 7 502 to 4 597.6 cm-1 after corrected by applying second derivative transformation and subtract a linear correction.Cross validation was used to prevent over-fitting.Good correlation existed between pinoresinol diglucoside content and NIR spectra（R2=0.926 4,SEC=0.029 and SEP=0.066 2）.Conclusion： NIRS calibration equations developed in this study could be applied to the rapid analysis of the pinoresinol diglucoside content.

关 键 词：近红外 杜仲 松脂醇二葡萄糖苷 偏最小二乘法 

分 类 号：R284.1[医药卫生—中药学]