Cox7a2通过ROS调控睾丸Leydig细胞睾酮生成的研究  

Regulation of testosterone production of Leydig cells by activity of ROS induced by Cox7a2

作　　者：钟伟[1] 陈亮[1] 贾金铭[2] 王明晓[1] 辛钟成[3] 郭应禄[3] 

机构地区：[1]国家安全生产监督管理总局煤炭总医院,北京100028 [2]中国中医科学院广安门医院 [3]北京大学第一医院

出　　处：《中国男科学杂志》2010年第11期14-18,共5页Chinese Journal of Andrology

基　　金：煤炭总医院科研课题资助项目(2009-02;2010-12)

摘　　要：目的研究Cox7a2调控睾酮生成的影响及其机制。方法构建Cox7a2荧光表达载体,转染TM3小鼠睾丸Leydig细胞。ELISA法测定上清液中睾酮含量,Western blot检测类固醇合成快速调节蛋白StAR表达,荧光分光光度计检测ROS水平。结果 Cox7a2抑制LH诱导的睾酮合成,降低StAR蛋白的表达,升高ROS水平。结论 Cox7a2通过抑制类固醇合成快速调节蛋白StAR的表达调控睾酮分泌,至少和增高ROS水平有关。Objective To investigate the regulation mechanism of steriodogenesis in Leydig cells by Cox7a2. Methods The recombinant expression vector pDsRed-Expression-N1-Cox7a2 was constructed and transfected into Leydig cells for Cox7a2 overexpression Level of testosterone in supernatant culture medium was measured by ELISA. The expression of steriodogenic acute regulatory protein （STAR） and reactive oxygen species （ROS） were detected by western blot and fluorometry. Results The recombinant expression vector pDsRed-Expression-N1-Cox7a2 was construct and transfected into Leydig cells suceesfully, Cox7a2 inhibited testosterone secretion of Leydig cells induced by LH treatment, and inhibited the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in Leydig cells. Conclusion Cox7a2 inhibited testosterone production of Leydig cells patially due to ROS activity increase and down-regulation of StAR protein.

关 键 词：衰老 COX7A2 睾酮 活性氧 

分 类 号：R697.2[医药卫生—泌尿科学] R394.33[医药卫生—外科学]