嗜酸细胞趋化因子在哮喘、嗜酸细胞性支气管炎患者诱导痰中的表达及其与气道炎症的关系  

Eotaxin in induced sputum in patients with asthma and eosinophilic bronchitis:relationship with airway inflammation

作　　者：付杰伟 蒋明彦 欧阳繁 

机构地区：[1]湘潭市中心医院呼吸内科,湖南湘潭411100

出　　处：《临床肺科杂志》2011年第1期33-34,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨嗜酸细胞趋化因子(eotaxin)在支气管哮喘、嗜酸细胞性支气管炎患者气道炎症发生中的作用以及在两者间的区别。方法分别收集支气管哮喘(A组)、嗜酸细胞性支气管炎(EB组)、单纯慢性支气管炎(CB组)缓解期/稳定期患者13例、11例、12例和健康对照者(C组)9例。诱导痰并经处理后进行分类细胞记数,并用夹心法酶联免疫吸附测定(ELISA)其诱导痰上清液中eotaxin浓度。结果诱导痰嗜酸细胞占白细胞百分比(Eos/Leu%)、eotaxin浓度A组[(19.2±9.7)%;(0.251±0.118)g/L]、EB组[(11.4±6.1)%;(0.146±0.079)g/L]分别与CB组[(0.9±0.6)%;(0.043±0.036)g/L]、C组[(0.8±0.4)%;(0.031±0.013)g/L]比较,差异有显著性(P均<0.05);A组Eos/Leu%、eotaxin浓度与EB组比较,差异也有显著性(P均<0.05)。结论 eotaxin可能通过对Eos的选择性趋化作用参与了支气管哮喘和嗜酸细胞性支气管炎患者气道炎症的发生机制,这种作用机制对于支气管哮喘和嗜酸细胞性支气管炎在程度上可能是不同的。Objective Investigate the roles of eotaxin（chemotactic factor of eosinophils）in pathogenesis of eosinophilic airway inflammation and the differences between ashma and eosinophilic brochitis.Methods 45 patients in stable phase with asthma（A group,13 cases）,eosinophilic bronchitis（EB group,11 cases）,chronic bronchitis（CB group,12 cases） and and 9 healthy volunteers as controls（C group）had participated in sputum induction.The concentration of eotaxin in supernatant was measured by sandwich enzyme linked immunosorbent assay（ELISA）.Results The differences of the eosinophil（Eos）/leukocyte（Leu）% and the concentration of eotaxin in A group[（19.2±9.7）%;（0.251±0.118）g/L],EB group[（11.4±6.1）%;（0.146±0.079）g/L]between in CB group[（0.9±0.6） %;（0.043±0.036）g/L],C group[（0.8±0.4）%;（0.031±0.013）g/L]are significant（P〈0.05）.The differences of those in A group between in EB group are significant too（P〈0.05）.Conclusion Eotaxin possiblely particulates in eosinophilic airway inflammation in patients with ashma and eosinophilic bronchitis by selective chemotactic activity on eosinophil,but it possiblely reachs to higher level in ashma than in eosinophilic bronchitis.

关 键 词：支气管哮喘 嗜酸细胞性支气管炎 诱导痰 气道炎症 嗜酸细胞趋化因子 

分 类 号：R562.25[医药卫生—呼吸系统]