内生真菌代谢产物球毛壳菌素A的液相色谱分析  

Analysis of Chaetoglobosin A Derived from Endophytic Fungus by HPLC

作　　者：程敬丽[1] 阮璐璐[1,2] 陆一夫[1,2] 章初龙[1] 

机构地区：[1]浙江大学农药与环境毒理研究所农业部作物病虫分子生物学开放重点实验室,杭州310029 [2]浙江工业大学化学工程与材料学院,杭州310032

出　　处：《农药》2011年第1期43-45,共3页Agrochemicals

基　　金：浙江省教育厅项目(Y200804493)

摘　　要：[目的]内生真菌代谢产物球毛壳菌素A具有优异、广谱的抑菌活性，具备良好的新型杀菌剂的开发前景。为了更好的分析发酵过程地液中球毛壳菌素A的含量，采用反相高效液棚色谱法，建立了内生真菌代谢产物球毛壳菌素A定量分析方法。[结果]经梯度洗脱优化出的色谱条件：使用DuPont ZORBAX ODS C18色谱柱，以乙腈为流动相A、水为流动相B，梯度条件：（A）0～10min，20％-25％；10～20min，25％-40％；20—55min，40％～55％；55-75min，55％～75％；75～85min，75％～90％。流速为1．0mL／min，柱温：25℃，波长为254nm。[结论]该方法精密度为1．49％，重现性为5．41％，可以作为球毛壳菌发酵优化过程中代谢产物球毛菌素A的检测技术。[Aims] Chaetoglobosin A have good broad-spectrum bactericidal activity, which show good development prospects for the new fungicides. Here, the quantitative analysis method of chaetoglobosin A derived from endophytic fungus was developed using reversed-phase high performance liquid chromatography（RP-HPLC）. [Results] The chromatographic conditions were optimized and obtained as follows： the separation was performed on a DuPont ZORBAX ODS C18 column. Acetonitrile（phase A） and ultrapure water（phase B） were used as mobile phase for gradient elution at a flow of 1.0 mL/min. The linear gradient of A was at 20-25%（0-10 min）, 25-40%（10- 20 min）, 40-55%（20-55 min）, 55-75%（55-75 min）, 75-90%（75-85 min）, respectively. The column temperature was kept at 25℃ and the detection wavelength was 254 nm. [Conclusions] The method showed that the standard derivation was 1.49% and repeatability was 5.41%. Using the chromatographic conditions, further optimization of the metabolites of chaetomium fermentation process of detection was investigated.

关 键 词：球毛壳菌素A 高效液相色谱 定量分析 

分 类 号：S482.2[农业科学—农药学]