Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA  

作　　者：Jie Hua Ke Long Huang 

机构地区：[1]School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China [2]School of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414000, China

出　　处：《Chinese Chemical Letters》2010年第12期1453-1456,共4页中国化学快报（英文版）

摘　　要：The separation of enzymatic hydrolysis products of yeast RNA by ion-pair HPLC was studied.A modified chromatographic response function（MCRF） was proposed to appraise the effectiveness of chromatographic separation.This function takes the number of peaks,resolution and the retention time of the last peak into consideration.It shows advantages for optimization of HPLC separation of complex mixtures.An orthogonal array design was used to separate the hydrolysate of yeast RNA and the optimal chromatographic conditions were obtained.The separation of enzymatic hydrolysis products of yeast RNA by ion-pair HPLC was studied.A modified chromatographic response function（MCRF） was proposed to appraise the effectiveness of chromatographic separation.This function takes the number of peaks,resolution and the retention time of the last peak into consideration.It shows advantages for optimization of HPLC separation of complex mixtures.An orthogonal array design was used to separate the hydrolysate of yeast RNA and the optimal chromatographic conditions were obtained.

关 键 词：Chromatographic response function Orthogonal array design 5＇-Phosphodiesterase Barley roots NUCLEOTIDES 

分 类 号：O522[理学—高压高温物理] TQ577.13[理学—物理]