Study on Modified Shengmai Yin Injection for Prevention and Treatment of Brain Impairment in Endotoxin Shock Rats  

作　　者：张宇忠 吴海燕 任丽薇 张宏生 贾旭 张银柱 

机构地区：[1]College of Preclinical Medicine, Beijing University of Chinese Medicine [2]Administrative Office of Experiment and Equipment, Beijing University of Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2010年第4期272-277,共6页中医杂志（英文版）

基　　金：supported by a grant from National Natural Science Foundation of China (No.30672737)

摘　　要：Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.Objective：To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods： Ninety-six SD rats were randomly divided into four groups： sham operation （saline 20 ml/kg）, shock model （lipopolysaccharide, LPS, 8 mg/kg）, Reformed Shengmai Yin （加味生脉饮 Pulse-activating Decoction） （LPS 8 mg/kg ＋ reformed Shengmai Yin Injection 10 ml/kg）, and dexamethasone （LPS 8 mg/kg ＋ dexamethasone 5 mg/kg） groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure （MAP）. Brain water levels, malonaldehyde （MDA） content, and superoxide dismutase （SOD） activity were also determined. Results： In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion： Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.

关 键 词：endotoxin shock brain impairment invigorate vital energy  promote blood flow  and detoxify free radicals Shengmai Yin （Pulse-activating Decoction） 

分 类 号：R285.5[医药卫生—中药学]