电针对慢性炎性反应疼痛大鼠血清TNF-α、IL-1、IL-4含量的影响  

Effects of Electroacupuncture of Unilateral and Bilateral "Zusanli" (ST36) on Serum TNF-α,IL-1 and IL-4 Levels in Rats with Chronic Inflammatory Pain

作　　者：王薇钧[1] 卢峻[1] 牛川森[2] 黄怡然[1] 马青[1] 阿英格[3] 郝红伟[2] 李路明[2] 图娅[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]清华大学宇航学院,北京100084 [3]华北煤炭医学院,唐山063000

出　　处：《针刺研究》2010年第6期429-432,共4页Acupuncture Research

基　　金：国家"863"课题重大研究资助项目(2006AA02Z4E9)

摘　　要：目的:观察电针刺激对炎性反应疼痛大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-4(IL-4)含量的影响,探讨电针刺激治疗慢性炎性反应疼痛的机制。方法:SD大鼠,随机分为4组:正常组、模型组、单穴电针组、双穴电针组,大鼠后肢足跖皮内注射弗氏完全佐剂复制炎性反应模型。单穴电针组、双穴电针组取"后三里"穴,每次电针30 min,每3 d治疗1次。35 d后取血清,以酶联免疫方法检测大鼠血清TNF-α、IL-1、IL-4的含量。结果:模型组大鼠血清TNF-α、IL-1、IL-4含量较正常组均明显升高(P<0.01),单穴电针组和双穴电针组大鼠血清TNF-α、IL-1、IL-4含量较模型组降低(均P<0.01),单穴电针组与双穴电针组各指标的差异均无统计学意义(P>0.05)。结论:单穴电针与双穴电针均可降低大鼠血清中TNF-α、IL-1、IL-4的含量,从而缓解机体的炎性反应,二者的作用效果无显著差异。Objective To observe the effect of electroacupuncture （EA） on serum tumor necrosis factor-α（TNF-α）, IL-1 and IL-4 levels in rats with chronic inflammatory pain, so as to investigate its underlying mechanism in relieving pain. Methods Forty-eight SD rats were randomized into control, model, EA-unilateral ST 36, and EA-bilateral ST 36 groups, with 12 rats in each. Chronic inflammatory pain model was established by intradermal injection of Freund＇s complete adjuvant （ 0. 1 mL） into the right paw. EA （2 Hz/100 Hz, 1-2-3 mA）was applied to the unilateral ＂Zusanli＂ （ST 36） and bilateral ＂Zusanli＂ （ST 36） for 30 min, once every 3 days and continuously for 4 weeks. Serum TNF-α, IL-1 and IL-4 contents were detected by enzyme linked immunosorbent assay. Results Compared with the control group, serum TNF-α, IL-1 and IL-4 contents of the model group increased significantly （P〈0.01）, while in comparison with the model group, the levels of the 3 indexes were decreased considerably in both unilateral and bilateral ST 36 groups （P〈0.01）. No significant differences were found between the unilateral ST 36 and bilateral ST 36 groups in serum TNF-α, IL-1 and IL-4 contents （P〈0.05）. Conclusion EA can effectively lower serum TNF-α, IL-1 and IL-4 levels in inflammatory pain rats, which may contribute to its effect in relieving inflammatory pain.

关 键 词：炎性反应疼痛 电针 肿瘤坏死因子-Α 白细胞介素-1 白细胞介素-4 

分 类 号：R245.97[医药卫生—针灸推拿学]