黄芪多糖对糖尿病足部溃疡成纤维细胞增殖功能的影响  

Effects of astragalus polysaccharides on the proliferation of fibroblast derived from diabetic foot ulcers

作　　者：肖正华[1] 张正军[1] 陈定宇[1] 周倩[1] 

机构地区：[1]广州市第一人民医院内分泌科,510180

出　　处：《中国糖尿病杂志》2010年第12期913-916,共4页Chinese Journal of Diabetes

基　　金：广东省科技厅社会发展领域科技计划项目(2003-245);广州市科技局应用基础研究项目(2006J1-C0261)

摘　　要：目的探讨黄芪多糖(APS)能否恢复糖尿病足部溃疡成纤维细胞(Fib)的增殖功能。方法以糖尿病急性伤口为对照,在了解糖尿病足部溃疡伤口炎症因子白介素1β(IL-1β)变化的基础上,进而探讨APS对溃疡处IL-1β含量的变化及Fib增殖功能的影响。结果 (1)糖尿病足溃疡患者创面渗出液中IL-1β含量为(89.16±38.14)ng/ml,糖尿病急性损伤患者创面渗出液中IL-1β含量为(8.84±7.06)ng/ml,两者比较,差异有统计学意义(P<0.01)。予黄芪多糖500μg/ml外敷足部溃疡14 d后IL-1β降至(11.67±3.12)ng/ml,与治疗前相比有显著差异(P<0.01),而与急性伤口相比却差异不显著(P>0.05)。(2)IL-1β(0.5～5 ng/ml)浓度范围可明显促进Fib增殖,随着浓度增加,50 ng/ml IL-1β对Fib增殖没有影响(P>0.05).IL-1β增加到500 ng/ml则对Fib增殖抑制作用(3)APS100μg/ml(vs IL-1β 50ng/ml组,P=0.003 vs 对照组P=0.002)和APS500 μg/ml(vs IL-1β 50 ng/ml组P=0.000,vs对照组P=0.000)可明显促进Fib增殖。结论 IL-1β在低浓度(0.5～5 ng/ml)对成纤维细胞的增殖有促进作用,当浓度增到50 ng/ml时对细胞增殖没有刺激作用。随着浓度进一步升高(500 ng/ml)时对细胞增殖有明显抑制作用。APS在100μg/ml以上浓度可显著刺激糖尿病足部溃疡部位成纤维细胞的增殖。这种作用有可能是通过降低渗出液中IL-1β浓度实现的。Objective To inquire into the effects of astragalus polysaccharides （ APs ）on proliferation of dermal fibroblasts（Fib） from human diabetic foot ulcers. Methods The wound oozing fluids from acute traumatic lesions 2 days after the injury were collected as a control. After the external application therapy with APs 500μg/ml, analysis of wound fluid IL 1β was performed by using ELISA. The fibrohlast cells from the edge of diabetic foot ulcers were dissociated, cultured, passaged and identified. After serum starvation for 24 hours, Fib cells were incubated for 12 h, 24 h, 48 h with IL-1β at o. 5 ng/ml, 5 ng/ml, 50 ng/ml, 500 ng/ml final concentrations and solvent 1640 media alone as control. The cells were divided into 5 groups： 1, control; 2, IL- 1β group（IL-1μ 50 ng/ml ）; 3, APs 20 μg/ml group（APs 20 μg/ml ）; 4, APs 100μg/ml group（APs 100 μg/ml ）; 5, APs 500 μg/ml group（APs 500 μg/ml ）. The cell growth curve was recorded by MTT. Results （1） The concentration of IL-1β in wound oozing fluid from diabetic foot ulcers was higher than that in acute diabetic traumatic lesions [（89.16±38.14） ng/ml versus （8.84±7.06） ng/ml, P〈0.01]. After treatment with APs 500μg/ml by external application, the concentration of IL-1β in wound oozing fluid from diabetic foot ulcers dropped to 11.67±3.12 ng/ml（P〈0. 01, vs Pre-therapy; P〉0. 05 vs Control）. （2）Compared with the control group, IL 1β at 0.5 ng/ml 5 ng/ml significantly stimulated Fibs proliferation, but had no such effect at 50 μg/ml. or could inhibit proliferation of Fbs at 500 ng/ml. Conclusions （1） The concentration of IL-1β is higher in wound oozing fluid from chronic diabetic foot ulcers than from acute diabetic traumatic wounds. （2） IL-1β can stimulate significantly Fbs proliferation at the concentration of 0.5 ng/ml-5 ng/ml with no effect at 50 ng/ml and it shows an inhibitive effect at 500 ng/ml. （3） APs can promote Fbs proliferation significantly.

关 键 词：糖尿病足部溃疡 黄芪多糖 成纤维细胞 白介素1Β 

分 类 号：R587.2[医药卫生—内分泌]