基于G-四联体的纳米探针比色检测铅离子  

Colorimetric Detection of Pb^(2+) Based on G-Quartet Structure by Nanoprobes

作　　者：莫志宏[1] 高应梅[1] 温志渝[1] 范艳萍[1] 

机构地区：[1]重庆大学化学化工学院,微系统研究中心,新型微纳器件与系统技术国防重点学科实验室,重庆400044

出　　处：《高等学校化学学报》2010年第11期2181-2183,共3页Chemical Journal of Chinese Universities

基　　金：国家“八六三”计划项目(批准号:2007AA042101);科技部国际科技合作项目(批准号:2007DFC00040)资助

摘　　要：基于纳米探针和G-四联体建立了简便快速检测铅离子的方法.纳米探针采用金纳米粒子自组装修饰富G寡核苷酸制得,在铅离子存在下,纳米探针上的富G寡核苷酸形成G-四联体,导致纳米探针凝聚变色.在优化条件下,比色检测铅离子的线性范围为48～480 nmol/L,检出限为20 nmol/L;大多数金属离子无明显干扰,而有明显干扰的汞离子可采用与之特异结合的寡核苷酸有效消除.将该法成功用于环境水样中铅离子的检测,重现性(RSD<3.0%)与回收率(98.4%～101.5%)良好.A simple and sensitive detection method for lead ions was developed,based on the G-quartet and nanoprobe.The nanoprobe was prepared by modification of gold nanoparticles with G-rich oligonucleotides in a self-assembled way.In the presence of Pb2 ＋,G-quartets are formed by G-rich oligonucleotides on the nanoprobes and result in the aggregation of nanoprobes and a change in color.Under the optimal condition of 200 mmol/L NaCl,2.0 mmol/L MgCl2 and at 37 ℃,the linear range was 48—480 nmol/L with a detection limit of 20 nmol/L for Pb2 ＋.Most metal ions have no interferences,and the interfering Hg2 ＋ can be effective-ly eliminated by a specific oligonucleotide.This method was applied for the detection of Pb2 ＋ in environmental water samples,with a good reproducibility（RSD 3.0%） and recovery（98.4% —101.5%）.

关 键 词：纳米探针 G-四联体 比色检测 铅离子 

分 类 号：O657[理学—分析化学]