大鼠脊髓源神经干细胞与运动神经元的差异蛋白质组学(英文)  

Comparison of differential proteomics between neural stem cells and motor neurons in embryonic spinal cord in rats

作　　者：王春芳[1] 李鹏飞[1] 蔚洪恩[1,2] 王菲[1] 韩树峰[3] 王树党[1] 窦岩[1] 赵嘉惠[1] 

机构地区：[1]山西医科大学生物技术教研室,太原030001 [2]第二军医大学解剖学教研室,上海200433 [3]山西医科大学第一附属医院,太原030001

出　　处：《解剖学报》2010年第6期779-784,共6页Acta Anatomica Sinica

基　　金：National Natural Science Foundation of China(30672114)

摘　　要：目的探讨大鼠脊髓源神经干细胞与运动神经元的差异蛋白质组学,寻找出重要的差异蛋白质。方法采用双向电泳分离两种细胞的蛋白质,用DeCyder软件分析蛋白表达差异,并采用质谱(HPLC-ESI-MS/MS)进行鉴定。结果脊髓源神经干细胞与运动神经元蛋白质凝胶分析获得1 300余个清晰的蛋白质斑点,在比国产分析的87个差异点中,初步鉴定出44个差异表达蛋白,其中24个在神经干细胞高表达,20个在运动神经元高表达。结论脊髓源神经干细胞与运动神经元有各自特定的蛋白质表达,某些差异蛋白可能在神经干细胞向运动神经元分化中发挥关键作用。Objective To analysis and compare the proteomic differences between neural stem cells and motor neurons in embryonic spinal cord in rat and discover the key different proteins. Methods Separating the protein of cells by the 2-D fluorescence difference gel electrophoresis, and to analyse the differences of protein expression with DeCyder software, and to identify with high performance liquid chromatography-electrospray tandem mass spectrometry. Results About 1 300 protein spots from the cells were gained after gel analysis. Eighty-seven protein spots were selected for mass spectrometry analysis. Fourty-four differently expressed proteins （24 in neural stem cells and 20 in motor neurons） were identified by mass spectrometry analysis.Conclusion Differently expressed proteins between neural stem cells and motor neurons were identified and it is helpful to find the new targets in neural stem cells differentiation into motor neurons.

关 键 词：神经干细胞 运动神经元 脊髓 蛋白质组学 大鼠 

分 类 号：R339.35[医药卫生—人体生理学]